Human Creatine Kinase B (CK-BB) CLIA Kit | CK-BB clia kit

Human Creatine Kinase B (CK-BB) CLIA Kit

Cat. Num. AAA2709035

• Gene Names: CKB; BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Creatine Kinase B (CK-BB); Human Creatine Kinase B (CK-BB) CLIA Kit; Creatine Kinase B; CKB; CKBB; CK-B; CK-1; Creatine Kinase; Brain; CK-BB clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Creatine Kinase B (CK-BB).; No significant cross-reactivity or interference between Creatine Kinase B (CK-BB) and analogues was observed.
• Sequence Length: 381

• Applicable Applications for CK-BB clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Detection Range: 0.27-200ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.11ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase B (CK-BB) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase B (CK-BB) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase B (CK-BB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Creatine Kinase B (CK-BB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Creatine Kinase B (CK-BB) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 125294
• NCBI GeneID: 1152
• UniProt Accession #: P12277
• NCBI Official Full Name: Creatine kinase B-type
• NCBI Official Synonym Full Names: creatine kinase B
• NCBI Official Symbol: CKB
• NCBI Official Synonym Symbols: BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• NCBI Protein Information: creatine kinase B-type
• UniProt Protein Name: Creatine kinase B-type
• UniProt Gene Name: CKB
• UniProt Synonym Gene Names: CKBB
• UniProt Entry Name: KCRB_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

CKB: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; creatine kinase activity; ATP binding

Biological Process: substantia nigra development; phosphorylation; creatine metabolic process; cellular chloride ion homeostasis