Typical Testing Data/Standard Curve (for reference only)
Human cPLA2 (Cytosolic Phospholipase A2) CLIA Kit | cPLA2 clia kit
Human cPLA2 (Cytosolic Phospholipase A2) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to cPLA2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for cPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain cPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of cPLA2. You can calculate the concentration of cPLA2 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Uniprot Description
cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.
Protein type: EC 3.1.1.5; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; EC 3.1.1.4; Phospholipase; Lipid Metabolism - ether lipid
Chromosomal Location of Human Ortholog: 1q25
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum membrane; Golgi apparatus; mitochondrial inner membrane; nucleus; perinuclear region of cytoplasm
Molecular Function: calcium ion binding; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; histone acetyltransferase binding; lysophospholipase activity; phospholipase A2 activity
Biological Process: aging; arachidonic acid metabolic process; arachidonic acid secretion; blood coagulation; decidualization; glycerophospholipid biosynthetic process; icosanoid biosynthetic process; icosanoid metabolic process; luteolysis; ovulation from ovarian follicle; phosphatidic acid biosynthetic process; phospholipid catabolic process; phospholipid metabolic process; platelet activating factor biosynthetic process; platelet activation; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell proliferation; positive regulation of fever; positive regulation of prostaglandin biosynthetic process; positive regulation of vesicle fusion; response to calcium ion; response to glucocorticoid stimulus; response to heat; response to hydrogen peroxide; response to lipopolysaccharide; response to lithium ion; response to methylmercury; response to organic nitrogen; response to vitamin D; surfactant homeostasis
Research Articles on cPLA2
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Product Notes
The Human cPLA2 pla2g4a (Catalog #AAA2532062) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532062 CLIA Kit recognizes Human cPLA2. It is sometimes possible for the material contained within the vial of "cPLA2 (Cytosolic Phospholipase A2), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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