Human Chemokine C-C-Motif Ligand 14 (CCL14) CLIA Kit | CCL14 clia kit

Human Chemokine C-C-Motif Ligand 14 (CCL14) CLIA Kit

Cat. Num. AAA2708899

• Gene Names: CCL14; CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Chemokine C-C-Motif Ligand 14 (CCL14); Human Chemokine C-C-Motif Ligand 14 (CCL14) CLIA Kit; Chemokine C-C-Motif Ligand 14; SCYA14; HCC-1; HCC-3; NCC-2; SCYL2; CKb1; MCIF; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 14; CCL14 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Ligand 14 (CCL14).; No significant cross-reactivity or interference between Chemokine C-C-Motif Ligand 14 (CCL14) and analogues was observed.
• Sequence Length: 93

• Applicable Applications for CCL14 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.54pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 14 (CCL14) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 14 (CCL14) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 14 (CCL14). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 14 (CCL14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-C-Motif Ligand 14 (CCL14) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 14589961
• NCBI GeneID: 6358
• NCBI Accession #: NP_116739.1
• NCBI GenBank Nucleotide #: NP_116739.1
• UniProt Accession #: Q16627
• NCBI Official Full Name: C-C motif chemokine 14 isoform 1
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 14
• NCBI Official Symbol: CCL14
• NCBI Official Synonym Symbols: CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• NCBI Protein Information: C-C motif chemokine 14
• UniProt Protein Name: C-C motif chemokine 14
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL14
• UniProt Synonym Gene Names: NCC2; SCYA14; HCC-1/HCC-3
• UniProt Entry Name: CCL14_HUMAN

NCBI Description

This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]

Uniprot Description

CCL14: Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cellular calcium ion homeostasis; positive regulation of cell proliferation; immune response