15-Hydroxyprostaglandin Dehydrogenase [NAD(+)]/HPGD Active Protein | HPGD active protein

Recombinant Human 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)]/HPGD Protein

Cat. Num. AAA2557065

• Gene Names: HPGD; PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)]/HPGD; Recombinant Human 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)]/HPGD Protein; 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)]; 15-PGDH; Prostaglandin Dehydrogenase 1; HPGD; PGDH1; HPGD active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 mum filtered solution of 20mM HEPES, 150mM NaCl, pH7.4.
• Sequence: Met 1-Gln266
• Sequence Length: 266

• Species: Human
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Fusion Tag: C-6His
• Activity: Measured by the production of NADH during the oxidation of PGF2alpha. The specific activity is >1,500 pmoles/min/mug.
• Preparation and Storage: Store at < -20 degree C, stable for 6 months. Please minimize freeze-thaw cycles.

Related Product Information for HPGD active protein

Protein Construction: Recombinant Human 15-Hydroxyprostaglandin Dehydrogenase [NAD(+)]/HPGD is produced by our mammalian expression system and the target gene encoding Met1-Gln266 is expressed with a 6His tag at the C-terminus.

Background: 15-hydroxyprostaglandin dehydrogenase [NAD(+)], also known as Prostaglandin dehydrogenase 1, 15-PGDH, HPGD and PGDH1, belongs to the short-chain dehydrogenases/reductases (SDR) family. HPGD localizes to the cytoplasm and can be found in colon epithelium, existing as a homodimer. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. HPGD is down-regulated by cortisol, dexamethasone and betamethasone, up-regulated by TGFB1. HPGD inhibits in vivo proliferation of colon cancer cells. HPGD is the key enzyme for the inactivation of prostaglandins, and thus regulates processes such as inflammation or proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 31542939
• NCBI GeneID: 3248
• NCBI Accession #: NP_000851.2
• NCBI GenBank Nucleotide #: NP_000851.2
• UniProt Accession #: P15428
• Molecular Weight: Molecular Mass: 30.0kDa; Actual Protein Molecular Mass: 29kDa
• NCBI Official Full Name: 15-hydroxyprostaglandin dehydrogenase
• NCBI Official Synonym Full Names: 15-hydroxyprostaglandin dehydrogenase
• NCBI Official Symbol: HPGD
• NCBI Official Synonym Symbols: PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1
• NCBI Protein Information: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• UniProt Protein Name: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• Protein Family: 15-hydroxyprostaglandin dehydrogenase
• UniProt Gene Name: HPGD
• UniProt Synonym Gene Names: PGDH1; 15-PGDH
• UniProt Entry Name: PGDH_HUMAN

NCBI Description

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

HPGD: Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of hypertrophic osteoarthropathy, primary, autosomal recessive, type 1 (PHOAR1). A disease characterized by digital clubbing, periostosis, acroosteolysis, painful joint enlargement, and variable features of pachydermia that include thickened facial skin and a thickened scalp. Other developmental anomalies include delayed closure of the cranial sutures and congenital heart disease. Defects in HPGD are the cause of cranioosteoarthropathy (COA). A form of osterarthropathy characterized by swelling of the joints, digital clubbing, hyperhidrosis, delayed closure of the fontanels, periostosis, and variable patent ductus arteriosus. Pachydermia is not a prominent feature. Defects in HPGD are a cause of isolated congenital nail clubbing (ICNC); also called clubbing of digits or hereditary acropachy. ICNC is a rare genodermatosis characterized by enlargement of the nail plate and terminal segments of the fingers and toes, resulting from proliferation of the connective tissues between the nail matrix and the distal phalanx. It is usually symmetrical and bilateral (in some cases unilateral). In nail clubbing usually the distal end of the nail matrix is relatively high compared to the proximal end, while the nail plate is complete but its dimensions and diameter more or less vary in comparison to normal. There may be different fingers and toes involved to varying degrees. Some fingers or toes are spared, but the thumbs are almost always involved. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; EC 1.1.1.141; Oxidoreductase

Chromosomal Location of Human Ortholog: 4q34-q35

Cellular Component: basolateral plasma membrane; cytosol

Molecular Function: protein homodimerization activity; 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; catalytic activity; NAD binding; prostaglandin E receptor activity

Biological Process: ovulation; lipoxygenase pathway; transforming growth factor beta receptor signaling pathway; cyclooxygenase pathway; arachidonic acid metabolic process; female pregnancy; parturition; negative regulation of cell cycle; prostaglandin metabolic process

Disease: Digital Clubbing, Isolated Congenital; Hypertrophic Osteoarthropathy, Primary, Autosomal Recessive, 1