Cardiac Troponin-T Native Protein | TNNT2 native protein

Human Cardiac Troponin-T

Cat. Num. AAA142897

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Cardiac Troponin-T; Human Cardiac Troponin-T; TnT Human; Cardiac Troponin T Human; Troponin T cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT; TNNT2; CMH2; CMPD2; MGC3889; Troponin-T; TNNT2 native protein

• Host: Human Cardiac Tissue
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: Human Cardiac Troponin T in 6M Urea, 250 mM NaCI, 50 mM Tris-HCI, 15mM Beta-mercaptoethanol, 1mM EDTA, pH 7.2
• Sequence Length: 295

• Physical Appearance: Sterile Filtered colorless liquid formulation (1.88 mg/mL).
• Human Virus Test: Blood samples from tissue donors used in the manufacture of this product tested negative for HIV-1,2, HbsAg, HCV and RPR/STS.
• Preparation and Storage: Human TnT although stable at 10°C for 7 days, should be stored desiccated below -18°C.; Please prevent freeze-thaw cycles.

Related Product Information for TNNT2 native protein

Description: Human Cardiac Troponin T produced from Human Cardiac Tissue, having a molecular weight of approximately 35 kDa.

The Cardiac troponin-T is purified using a combination of ion-exchange and affinity chromatography steps.

Introduction: Troponin-T is one of three subunits that form the troponin complex of the thin filaments of striated muscle, it regulates muscle contraction in response to alterations in intracellular calcium ion concentration. TnTc is expressed only in the heart and has proved to be a specific biomarker for heart disease. Mutations in TNNT2 are associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Alternative splicing results in many tissue-specific isoforms.

Product Categories/Family for TNNT2 native protein

Recombinant & Natural Proteins; Recombinant Proteins; Cardiac Troponin

References

Preliminary development of a DNA aptamer-magnetic bead capture electrochemiluminescence sandwich assay for brain natriuretic peptide. John G. Bruno, Alicia M. Richarte, Taylor Phillips (2014)

NCBI and Uniprot Product Information

• NCBI GI #: 48255877
• NCBI GeneID: 7139
• NCBI Accession #: NP_000355.2
• NCBI GenBank Nucleotide #: NM_000364.3
• UniProt Accession #: P45379; O60214; Q99596; Q99597; Q9BUF6; Q9UM96; A2TDB9; A8K3K6
• NCBI Official Full Name: troponin T, cardiac muscle isoform 1
• NCBI Official Synonym Full Names: troponin T type 2 (cardiac)
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle; cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; troponin T2, cardiac
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT2
• UniProt Synonym Gene Names: TnTc; cTnT
• UniProt Entry Name: TNNT2_HUMAN

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding

Biological Process: positive regulation of ATPase activity; regulation of muscle contraction; metabolic process; atrial cardiac muscle morphogenesis; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2