anti-Human Zyxin Antibody | anti-Zyxin antibody

Zyxin (Phospho-Ser142) Antibody

Cat. Num. AAA851664

• Gene Names: ZYX; ESP-2; HED-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: Zyxin; Zyxin (Phospho-Ser142) Antibody; ZYX; Zyxin 2; anti-Zyxin antibody

• Reactivity: Human
• Specificity: Zyxin (Phospho-Ser142) antibody detects endogenous levels of Zyxin only when phosphorylated at serine 142.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 572

• Applicable Applications for anti-Zyxin antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Zyxin around the phosphorylation site of serine 142 (K-V-SP-S-I).
• All Sites: Human: Ser142
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, treated with paclitaxel (1uM, 24hours), using Zyxin (Phospho-Ser142) antibody.)

Product Categories/Family for anti-Zyxin antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Macalma T., J. Biol. Chem. 271:31470-31478(1996). Zumbrunn J., Eur. J. Biochem. 241:657-663(1996). Hillier L.W., Nature 424:157-164(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 58530845
• NCBI GeneID: 7791
• NCBI Accession #: NP_001010972.1
• NCBI GenBank Nucleotide #: NM_001010972.1
• UniProt Accession #: Q15942; Q6I9S4; A4D2G6; B4DQX7
• Molecular Weight: 45,075 Da
• NCBI Official Full Name: zyxin isoform 1
• NCBI Official Synonym Full Names: zyxin
• NCBI Official Symbol: ZYX
• NCBI Official Synonym Symbols: ESP-2; HED-2
• NCBI Protein Information: zyxin
• UniProt Protein Name: Zyxin
• Protein Family: Zyxin
• UniProt Gene Name: ZYX

NCBI Description

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression ().