anti-Human TIAM1 Antibody | anti-TIAM1 antibody

Goat anti-TIAM1 Antibody

Cat. Num. AAA423697

• Gene Names: TIAM1; TIAM-1
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TIAM1; Goat anti-TIAM1 Antibody; T-cell lymphoma invasion and metastasis 1; human T-lymphoma invasion and metastasis inducing TIAM1 protein; anti-TIAM1 antibody

• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 1591

• Applicable Applications for anti-TIAM1 antibody: Peptide ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:128000.; IF: Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10ug/ml. ; Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Immunogen Sequence: Peptide with sequence C-EDFAPSRKLNTEI, from the C Terminus of the protein sequence according to NP_003244; NP_001340613.1; NP_001340614.1; NP_001340615.1; NP_001340616.1.
• Peptide Sequence + C: C-EDFAPSRKLNTEI
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 115583670
• NCBI GeneID: 7074
• NCBI Accession #: NP_003244; NP_001340613.1; NP_001340614.1; NP_001340615.1; NP_001340616.1
• NCBI GenBank Nucleotide #: NM_003253.3
• UniProt Accession #: Q13009; Q17RT7; B7ZLR6; F5GZ53
• Molecular Weight: 170,580 Da
• NCBI Official Full Name: T-lymphoma invasion and metastasis-inducing protein 1 isoform 1
• NCBI Official Synonym Full Names: T cell lymphoma invasion and metastasis 1
• NCBI Official Symbol: TIAM1
• NCBI Official Synonym Symbols: TIAM-1
• NCBI Protein Information: T-lymphoma invasion and metastasis-inducing protein 1
• UniProt Protein Name: T-lymphoma invasion and metastasis-inducing protein 1
• Protein Family: T-lymphoma invasion and metastasis-inducing protein
• UniProt Gene Name: TIAM1
• UniProt Synonym Gene Names: TIAM-1

NCBI Description

This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain. [provided by RefSeq, Jul 2017]

Uniprot Description

Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA. Required for normal cell adhesion and cell migration.