anti-Human SMG7 Antibody | anti-SMG7 antibody

SMG7 Antibody

Cat. Num. AAA856343

• Gene Names: SMG7; EST1C; SGA56M; C1orf16
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: SMG7; SMG7 Antibody; C1orf16; EST1C; KIAA0250; anti-SMG7 antibody

• Reactivity: Human
• Specificity: SMG7 antibody detects endogenous levels of total SMG7 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1145

• Applicable Applications for anti-SMG7 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human SMG7.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Western Blot (WB)

(Peptide-+ Western blot analysis of extracts from HeLa cells, Jurkat cells, 293 cells and HUVEC cells, using SMG7 antibody. Immunofluorescence analysis of HepG2 cells, using SMG7 antibody.)

Product Categories/Family for anti-SMG7 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Ohnishi T., Mol. Cell 12:1187-1200(2003). Nagase T., DNA Res. 3:321-329(1996). Nakajima D., DNA Res. 9:99-106(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 291327485
• NCBI GeneID: 9887
• NCBI Accession #: NP_001167532.1
• NCBI GenBank Nucleotide #: NM_001174061.1
• UniProt Accession #: Q92540; Q5T1Q0; Q6PIE0; Q7Z7H9; Q8IXC1; Q8IXC2; B4DRB2; E9PCI0; E9PEH2
• Molecular Weight: 127,854 Da
• NCBI Official Full Name: protein SMG7 isoform 5
• NCBI Official Synonym Full Names: SMG7, nonsense mediated mRNA decay factor
• NCBI Official Symbol: SMG7
• NCBI Official Synonym Symbols: EST1C; SGA56M; C1orf16
• NCBI Protein Information: protein SMG7
• UniProt Protein Name: Protein SMG7
• Protein Family: Protein
• UniProt Gene Name: SMG7
• UniProt Synonym Gene Names: C1orf16; EST1C; KIAA0250; hSMG-7

NCBI Description

This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

Uniprot Description

Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.