Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SKP2/p45 antibody.)
anti-Human SKP2/p45 Antibody | anti-SKP2/p45 antibody
SKP2/p45 Antibody
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SKP2/p45 antibody.)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SKP2/p45 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using SKP2/p45 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using SKP2/p45 antibody.)
Immunofluorescence (IF)
(Peptide-+ Immunofluorescence analysis of A549 cells, using SKP2/p45 antibody.)
Immunofluorescence (IF)
(Peptide-+ Immunofluorescence analysis of A549 cells, using SKP2/p45 antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2011]
Uniprot Description
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.
Research Articles on SKP2/p45
Similar Products
Product Notes
The SKP2/p45 skp2 (Catalog #AAA855486) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The SKP2/p45 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SKP2/p45 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the SKP2/p45 skp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SKP2/p45, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
