anti-Human PPM1A Antibody | anti-PPM1A antibody

PPM1A Antibody

Cat. Num. AAA8526888

• Gene Names: PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Protein G-Sepharose affinity purified.
• Synonyms: PPM1A; PPM1A Antibody; Mouse monoclonal IgG to PPM1A; anti-PPM1A antibody

• Reactivity: Human
• Purity/Purification: Protein G-Sepharose affinity purified.
• Form/Format: Liquid
• Sequence Length: 382

• Applicable Applications for anti-PPM1A antibody: ELSIA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:5000-1:20000; WB: 1:100-1:2000
• Immunogen: Recombinant fragment of human PPM1A (N-terminus).
• Preservative: 0.02% Sodium Azide and 50% glycerol, 1% BSA, PBS, pH7.4.
• Cellular Localization: Cytoplasm; Nucleus
• Preparation and Storage: Aliquot and store at-20 degree C. Avoid repeated freeze/thaw cycles.

Testing Data

(PPM1A (N-terminus) antibody at 1/1000 dilution + Hela whole cell lysate 40ug/Lane. Predicted band size: 42KDa. Observed band size: 42KDa.)

Related Product Information for anti-PPM1A antibody

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases.

NCBI and Uniprot Product Information

• NCBI GI #: 10337595
• NCBI GeneID: 5494
• NCBI Accession #: NP_066283.1
• NCBI GenBank Nucleotide #: NM_177952.1
• UniProt Accession #: P35813; O75551; B5BU11; J3KNM0
• Molecular Weight: 51,366 Da
• NCBI Official Full Name: protein phosphatase 1A isoform 1
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent 1A
• NCBI Official Symbol: PPM1A
• NCBI Official Synonym Symbols: PP2CA; PP2Calpha; PP2C-ALPHA
• NCBI Protein Information: protein phosphatase 1A
• UniProt Protein Name: Protein phosphatase 1A
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1A
• UniProt Synonym Gene Names: PPPM1A; PP2C-alpha

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.