anti-Human GPR149 Antibody | anti-GPR149 antibody

GPR149 Antibody

Cat. Num. AAA855446

• Gene Names: GPR149; R35; IEDA; PGR10
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: GPR149; GPR149 Antibody; GP149; anti-GPR149 antibody

• Reactivity: Human
• Specificity: GPR149 antibody detects endogenous levels of total GPR149 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 731

• Applicable Applications for anti-GPR149 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human GPR149.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Western Blot (WB)

(Peptide-+ Western blot analysis of extracts from HeLa cells and COLO205 cells, using GPR149 antibody. Immunofluorescence analysis of HeLa cells, using GPR149 antibody.)

Product Categories/Family for anti-GPR149 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Muzny D.M., Nature 440:1194-1198(2006). Vassilatis D.K., Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 84662753
• NCBI GeneID: 344758
• NCBI Accession #: NP_001033794.1
• NCBI GenBank Nucleotide #: NM_001038705.2
• UniProt Accession #: Q86SP6
• Molecular Weight: 80,984 Da
• NCBI Official Full Name: probable G-protein coupled receptor 149
• NCBI Official Synonym Full Names: G protein-coupled receptor 149
• NCBI Official Symbol: GPR149
• NCBI Official Synonym Symbols: R35; IEDA; PGR10
• NCBI Protein Information: probable G-protein coupled receptor 149
• UniProt Protein Name: Probable G-protein coupled receptor 149
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: GPR149
• UniProt Synonym Gene Names: PGR10

NCBI Description

This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017]

Uniprot Description

Orphan receptor.