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Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 antibody.)

anti-Human Cytochrome P450 27A1 Antibody | anti-CYP27A1 antibody

Cytochrome P450 27A1 Antibody

Gene Names
CYP27A1; CTX; CP27; CYP27
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Cytochrome P450 27A1; Cytochrome P450 27A1 Antibody; 25-hydroxylase; 5-beta-cholestane-3-alpha; 7-alpha; 12-alpha-triol 27-hydroxylase; CP27A; Cytochrome P-450C27/25; Cytochrome P450 27; mitochondrial; EC 1.14.13.15; Sterol 26-hydroxylase; Sterol 27-hydroxylase; Vitamin D; anti-CYP27A1 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Cytochrome P450 27A1 antibody detects endogenous levels of total Cytochrome P450 27A1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
531
Applicable Applications for anti-CYP27A1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human Cytochrome P450 27A1.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 antibody.)
References
Cali J.J., J. Biol. Chem. 266:7774-7778(1991). Guo Y.-D., Proc. Natl. Acad. Sci. U.S.A. 90:8668-8672(1993). Leitersdorf E., J. Clin. Invest. 91:2488-2496(1993).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,235 Da
NCBI Official Full Name
sterol 26-hydroxylase, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450 family 27 subfamily A member 1
NCBI Official Symbol
CYP27A1
NCBI Official Synonym Symbols
CTX; CP27; CYP27
NCBI Protein Information
sterol 26-hydroxylase, mitochondrial
UniProt Protein Name
Sterol 26-hydroxylase, mitochondrial
Protein Family
UniProt Gene Name
CYP27A1
UniProt Synonym Gene Names
CYP27

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.

Research Articles on CYP27A1

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Product Notes

The CYP27A1 cyp27a1 (Catalog #AAA852683) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 27A1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 27A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the CYP27A1 cyp27a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 27A1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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