Testing Data
(P-peptide-+)
anti-Human CDC25C Antibody | anti-CDC25C antibody
CDC25C (Phospho-Ser216) Antibody
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
CDC25C; CDC25C (Phospho-Ser216) Antibody; CDC25M1; dual specificity phosphatase Cdc25C; EC 3.1.3.48; M-phase inducer phosphatase 3; MPI1B; MPIP3; anti-CDC25C antibody
Reactivity
Human
Specificity
CDC25C (Phospho-Ser216) antibody detects endogenous levels of CDC25C only when phosphorylated at serine 216.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
473
Applicable Applications for anti-CDC25C antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
IHC: 1:50~1:100
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human CDC25C around the phosphorylation site of serine 216 (S-P-SP-M-P).
All Sites
Human: Ser216
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CDC25C (Phospho-Ser216) antibody. Western blot analysis of extracts from HUVEC cells, treated with serum (20%, 30mins), using CDC25C (Phospho-Ser216) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CDC25C (Phospho-Ser216) antibody. Western blot analysis of extracts from HUVEC cells, treated with serum (20%, 30mins), using CDC25C (Phospho-Ser216) antibody.)
References
Sadhu K., Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990). Bureik M., Int. J. Oncol. 17:1251-1258(2000). Livingston R.J., Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,608 Da
NCBI Official Full Name
M-phase inducer phosphatase 3 isoform a
NCBI Official Synonym Full Names
cell division cycle 25C
NCBI Official Symbol
CDC25C
NCBI Official Synonym Symbols
CDC25; PPP1R60
NCBI Protein Information
M-phase inducer phosphatase 3
UniProt Protein Name
M-phase inducer phosphatase 3
UniProt Gene Name
CDC25C
NCBI Description
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
Uniprot Description
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Research Articles on CDC25C
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Product Notes
The CDC25C cdc25c (Catalog #AAA8510097) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CDC25C (Phospho-Ser216) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC25C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the CDC25C cdc25c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC25C, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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