anti-Human CASP5 Antibody | anti-CASP5 antibody

CASP5 (p10, Cleaved-Ser331) Antibody

Cat. Num. AAA853717

• Gene Names: CASP5; ICH-3; ICEREL-III; ICE(rel)III
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CASP5; CASP5 (p10; Cleaved-Ser331) Antibody; Caspase-5 [Precursor]; EC 3.4.22.58; CASP-5; ICH-3 protease; TY protease; ICE(rel)-III; Caspase-5 subunit p20; Caspase-5 subunit p10; ICH3; anti-CASP5 antibody

• Reactivity: Human
• Specificity: CASP5 (p10, Cleaved-Ser331) antibody detects endogenous levels of fragment of activated CASP5 resulting from cleavage adjacent to Ser33.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 376

• Applicable Applications for anti-CASP5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from human CASP5.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 1hour), using CASP5 (p10, Cleaved-Ser331) antibody.)

References

Munday N.A., J. Biol. Chem. 270:15870-15876(1995). Faucheu C., Eur. J. Biochem. 236:207-213(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 209870071
• NCBI GeneID: 838
• NCBI Accession #: NP_001129581.1
• NCBI GenBank Nucleotide #: NM_001136109.2
• UniProt Accession #: P51878; Q0QVY7; Q0QVY8; Q0QVZ0; Q0QVZ1; Q0QVZ2; Q14DD6; Q1HBJ3; Q6DJV7; B4DKP5
• Molecular Weight: 41,851 Da
• NCBI Official Full Name: caspase-5 isoform b
• NCBI Official Synonym Full Names: caspase 5
• NCBI Official Symbol: CASP5
• NCBI Official Synonym Symbols: ICH-3; ICEREL-III; ICE(rel)III
• NCBI Protein Information: caspase-5
• UniProt Protein Name: Caspase-5
• Protein Family: Caspase
• UniProt Gene Name: CASP5
• UniProt Synonym Gene Names: ICH3; CASP-5

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

Mediator of programmed cell death (apoptosis). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).