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Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of 3T3 cells, using Breast Tumor Kinase antibody.)

anti-Human Breast Tumor Kinase Antibody | anti-PTK6 antibody

Breast Tumor Kinase Antibody

Gene Names
PTK6; BRK
Reactivity
Human
Applications
Immunofluorescence, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Breast Tumor Kinase; Breast Tumor Kinase Antibody; Breast tumor kinase; EC 2.7.10.2; PTK6; Tyrosine-protein kinase 6; Tyrosine-protein kinase brk; anti-PTK6 antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Breast Tumor Kinase antibody detects endogenous levels of total Breast Tumor Kinase protein.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
134
Applicable Applications for anti-PTK6 antibody
Immunofluorescence (IF), ELISA (EIA)
Application Notes
IF: 1:100-1:500
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human Breast Tumor Kinase.
Preparation and Storage
Store at-20 degree C for 1 year.

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of 3T3 cells, using Breast Tumor Kinase antibody.)

Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of 3T3 cells, using Breast Tumor Kinase antibody.)
References
Mitchell P.J., Oncogene 9:2383-2390(1994). Lee H.-Y., Mol. Cells 8:401-407(1998). Ota T., Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,465 Da
NCBI Official Full Name
protein-tyrosine kinase 6 isoform 2
NCBI Official Synonym Full Names
protein tyrosine kinase 6
NCBI Official Symbol
PTK6
NCBI Official Synonym Symbols
BRK
NCBI Protein Information
protein-tyrosine kinase 6
UniProt Protein Name
Protein-tyrosine kinase 6
Protein Family
UniProt Gene Name
PTK6
UniProt Synonym Gene Names
BRK

NCBI Description

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.MiscellaneousThe inhibitors bind to the ATP-binding pocket.

Research Articles on PTK6

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Product Notes

The PTK6 ptk6 (Catalog #AAA8509604) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Breast Tumor Kinase Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Breast Tumor Kinase can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA). IF: 1:100-1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the PTK6 ptk6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Breast Tumor Kinase, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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