anti-Human AP3B1/HPS Antibody | anti-AP3B1 antibody

Goat anti-AP3B1/HPS Antibody

Cat. Num. AAA423703

• Gene Names: AP3B1; PE; HPS; HPS2; ADTB3; ADTB3A
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: AP3B1/HPS; Goat anti-AP3B1/HPS Antibody; AP3B1; PE; HPS; HPS2; ADTB3; ADTB3A; adaptor-related protein complex 3; beta 1 subunit; beta3A-adaptin; AP-3 complex beta-3A subunit; anti-AP3B1 antibody

• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 1094

• Applicable Applications for anti-AP3B1 antibody: Peptide ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:64000.; FC/FACS: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
• Immunogen Sequence: Peptide with sequence SSNSFPYNEQSGG-C, from the N Terminus of the protein sequence according to NP_003655.
• Peptide Sequence + C: SSNSFPYNEQSGG-C
• Epitope: N Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 32484979
• NCBI GeneID: 8546
• NCBI Accession #: NP_003655
• NCBI GenBank Nucleotide #: NM_003664.4
• UniProt Accession #: O00203; O00580; Q7Z393; Q9HD66; E5RJ68
• Molecular Weight: 116,195 Da
• NCBI Official Full Name: AP-3 complex subunit beta-1 isoform 1
• NCBI Official Synonym Full Names: adaptor related protein complex 3 subunit beta 1
• NCBI Official Symbol: AP3B1
• NCBI Official Synonym Symbols: PE; HPS; HPS2; ADTB3; ADTB3A
• NCBI Protein Information: AP-3 complex subunit beta-1
• UniProt Protein Name: AP-3 complex subunit beta-1
• Protein Family: AP-3 complex
• UniProt Gene Name: AP3B1
• UniProt Synonym Gene Names: ADTB3A

NCBI Description

This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.