PDGF AA active protein

PDGF AA protein

Cat. Num. AAA539595

• Gene Names: PDGFA; PDGF1; PDGF-A
• Applications: User optimized
• Purity: > 98% pure
• Synonyms: PDGF AA; PDGF AA protein; GDGF protein; Platelet-Derived Growth Factor-AA protein; Glioma-derived growth factor protein; ODGF protein; Osteosarcoma-derived Growth Factor protein; PDGF AA active protein

• Host: Human
• Purity/Purification: > 98% pure
• Form/Format: Supplied as a lyophilized powder.
• Sequence: Region of PDGF AA protein corresponding to amino acids MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT.
• Sequence Length: 211

• Applicable Applications for PDGF AA active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-beta. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
• Bioactivity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 8-10 ng/ml.
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for PDGF AA active protein

Purified recombinant Human PDGF AA protein

Product Categories/Family for PDGF AA active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 129719
• NCBI GeneID: 5154
• UniProt Accession #: P04085; B5BU73
• Molecular Weight: 28.7 kDa
• NCBI Official Full Name: Platelet-derived growth factor subunit A
• NCBI Official Synonym Full Names: platelet-derived growth factor alpha polypeptide
• NCBI Official Symbol: PDGFA
• NCBI Official Synonym Symbols: PDGF1; PDGF-A
• NCBI Protein Information: platelet-derived growth factor subunit A
• UniProt Protein Name: Platelet-derived growth factor subunit A
• UniProt Gene Name: PDGFA
• UniProt Synonym Gene Names: PDGF1; PDGF subunit A
• UniProt Entry Name: PDGFA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cytokine; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extracellular space; microvillus; cell surface; endoplasmic reticulum lumen; extracellular region

Molecular Function: collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; platelet degranulation; regulation of smooth muscle cell migration; hair follicle development; cell-cell signaling; positive regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; inner ear development; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; fibroblast growth factor receptor signaling pathway; response to retinoic acid; phosphoinositide-mediated signaling; response to inorganic substance; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; cell activation; organ morphogenesis; regulation of actin cytoskeleton organization and biogenesis; positive regulation of cell division; response to hypoxia; innate immune response; blood coagulation; actin cytoskeleton organization and biogenesis; alveolus development; positive regulation of DNA replication; positive regulation of cell migration