JAK2, N-terminal GST tag Active Protein | jak2b active protein
JAK2, Recombinant, Human, N-terminal GST tag (Janus Kinase 2)
Gene Names
jak2b; jak2; JAK2bCA
Reactivity
Human
Purity
80% by SDS-PAGE
Synonyms
JAK2; N-terminal GST tag; Recombinant; Human; N-terminal GST tag (Janus Kinase 2); jak2b active protein
Reactivity
Human
Purity/Purification
80% by SDS-PAGE
Form/Format
25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.
Application Notes
useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity
4 U/ug. One unit is defined as the amount of enzyme that will incorporate 1 nmol phosphate to the tyrosine substrate per minute at pH 7.4 and 30 degree C
Assay Buffer
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 0.04ug recombinant JAK2
Preparation and Storage
-70 degree C
Stability: >6 months at -80 degree C
Stability: >6 months at -80 degree C
Related Product Information for jak2b active protein
The Janus (JAK) family of cytoplasmic tyrosine kinases has been implicated in signal transduction induced by cytokines and growth factors, physically associated with ligand-bound receptors. This association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130kD and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members. Family members such as JAK1, JAK2, and TYK2 are ubiquitous, whereas JAK3 is predominantly expressed in T lymphocytes. Studies with mutant cells that do not express JAK1, JAK2, or TYK2 show that their activation is essential for cellular signaling. JAK family kinases may either phosphorylate each other or be phosphorylated by a yet undescribed tyrosine kinase. Different cytokines can activate via phosphorylation distinct JAK family members. In some cells, the membrane-associated gp130 protein has been implicated in the induction of JAK family phosphorylation, although gp130 itself has no known activity.
Human Jak2 (GenBank Accession No. NM_004972), (a.a. 808-end) with N-terminal His tag
MW 42.5kD, expressed in an Baculovirus infected Sf9 cell expression system.
Human Jak2 (GenBank Accession No. NM_004972), (a.a. 808-end) with N-terminal His tag
MW 42.5kD, expressed in an Baculovirus infected Sf9 cell expression system.
Product Categories/Family for jak2b active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42.5kD
NCBI Official Full Name
jak2
NCBI Official Synonym Full Names
Janus kinase 2b
NCBI Official Symbol
jak2b
NCBI Official Synonym Symbols
jak2; JAK2bCA
NCBI Protein Information
tyrosine-protein kinase JAK2; PTK JAK2b; Jak2b kinase; protein tyrosine kinase JAK2b
Research Articles on jak2b
Similar Products
Product Notes
The jak2b (Catalog #AAA637772) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The JAK2, Recombinant, Human, N-terminal GST tag (Janus Kinase 2) reacts with Human and may cross-react with other species as described in the data sheet. useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Researchers should empirically determine the suitability of the jak2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAK2, N-terminal GST tag, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
