IL10 active protein

IL10 protein

Cat. Num. AAA5304240

• Gene Names: IL10; CSIF; TGIF; GVHDS; IL-10; IL10A
• Purity: > 98% pure; IL10 protein was purified using a a proprietary chromatographic technique.
• Synonyms: IL10; IL10 protein; TGIF protein; IL-10 protein; IL 10; Cytokine synthesis inhibitory factor protein; IL-10; MGC126451 protein; MGC126450 protein; IL 10 protein; CSIF protein; IL10A protein; B-TCGF protein; IL10 active protein

• Host: Human
• Purity/Purification: > 98% pure; IL10 protein was purified using a a proprietary chromatographic technique.
• Form/Format: Supplied in lyophilized form from PBS
• Sequence Length: 178

• Protein Type: Recombinant
• Biological Significance: IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.
• Bioactivity: The ED50 determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 10^5 units/mg
• Endotoxin Levels: <0.1 ng/ug
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for IL10 active protein

Purified recombinant Human IL10 protein

Product Categories/Family for IL10 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 49457009
• NCBI GeneID: 3586
• UniProt Accession #: Q6FGS9; P22301
• Molecular Weight: 18.6 kDa
• NCBI Official Full Name: IL10, partial
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: IL10
• NCBI Official Synonym Symbols: CSIF; TGIF; GVHDS; IL-10; IL10A
• NCBI Protein Information: interleukin-10
• UniProt Protein Name: IL10 protein
• Protein Family: Interleukin
• UniProt Gene Name: IL10
• UniProt Entry Name: Q6FGS9_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding

Biological Process: B cell proliferation; regulation of isotype switching; negative regulation of chronic inflammatory response to antigenic stimulus; leukocyte chemotaxis; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; cytoplasmic sequestering of NF-kappaB; negative regulation of T cell proliferation; cell-cell signaling; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor biosynthetic process; hemopoiesis; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; T-helper 2 type immune response; positive regulation of B cell apoptosis; negative regulation of nitric oxide biosynthetic process; negative regulation of myeloid dendritic cell activation; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; regulation of sensory perception of pain; response to activity; receptor biosynthetic process; negative regulation of apoptosis; positive regulation of cytokine secretion

Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To