IGF BP3 active protein

IGF BP3 protein

Cat. Num. AAA539422

• Gene Names: IGFBP3; IBP3; BP-53
• Applications: User optimized
• Purity: > 98% pure
• Synonyms: IGF BP3; IGF BP3 protein; IGF BP-3 protein; Insulin-like Growth Factor-Binding Protein 3 protein; Growth-hormone-dependant binding protein protein; IGF BP-3; IGF BP 3 protein; IGF BP 3; IGF BP3 active protein

• Host: Human
• Purity/Purification: > 98% pure
• Form/Format: Lyophilized from 20 mM NaCl. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml.
• Sequence: Region of IGF BP3 protein corresponding to amino acids GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK.
• Sequence Length: 297

• Applicable Applications for IGF BP3 active protein: User optimized
• Protein Type: Recombinant
• Biological Significance: IGF-BP3 is a 30 kDa cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals and it is also found in a-granules of platelets. In addition to its ability to modulate the activity of IGF-I and IGF-II, IGF-BP3 exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF, and EGF.
• Bioactivity: The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is approximately 0.2 ug/ml in presence of 15 ng/ml of human IGF-II.
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for IGF BP3 active protein

Purified recombinant Human IGF BP3 protein

Product Categories/Family for IGF BP3 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 62243248
• NCBI GeneID: 3486
• NCBI Accession #: NP_001013416.1
• NCBI GenBank Nucleotide #: NM_001013398.1
• UniProt Accession #: P17936; Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0
• Molecular Weight: 28.8 kDa
• NCBI Official Full Name: insulin-like growth factor-binding protein 3 isoform a
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: IGFBP3
• NCBI Official Synonym Symbols: IBP3; BP-53
• NCBI Protein Information: insulin-like growth factor-binding protein 3
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• UniProt Gene Name: IGFBP3
• UniProt Synonym Gene Names: IBP3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region; nucleus

Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; metal ion binding; fibronectin binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity

Biological Process: positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; regulation of insulin-like growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of smooth muscle cell proliferation; apoptosis; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; negative regulation of signal transduction; osteoblast differentiation; negative regulation of cell proliferation; cellular protein metabolic process; positive regulation of MAPKKK cascade; negative regulation of protein amino acid phosphorylation; regulation of cell growth