GCSF active protein

GCSF protein

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Applications: Western Blot
• Purity: > 95% pure
• Synonyms: GCSF; GCSF protein; Colony stimulating factor 3 (granulocyte) protein; CSF 3 protein; CSF beta protein; CSF3 protein; Csfg protein; Filgrastim protein; GCSA protein; Granulocyte colony stimulating factor protein; Lenograstim protein; Macrophage granulocyte inducer 2 protein; MGC45931 protein; MGI 2 protein; Pluripoietin protein; GCSF active protein

• Host: Human
• Purity/Purification: > 95% pure
• Form/Format: Supplied in liquid form in PBS, 10% glycerol
• Concentration: 0.5mg/ml (varies by lot)
• Sequence Length: 168

• Applicable Applications for GCSF active protein: Western Blot (WB)
• Protein Type: Recombinant
• Biological Significance: Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic cytokine that promotes the proliferation and differentiation of cells in the granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cells can also secrete G-CSF after IL-1b,TNF-alpha and LPS activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF has a effect in the treatment of leukopenia, AIDS, MDS and bone marrow transplantation. In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both protein have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein.
• Bioactivity: The ED50 of G-CSF is typically 5-40 pg/mL. Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
• Expression System: E Coli
• Preparation and Storage: Store at -80 degree C. Avoid frequent freeze/thaw cycles

Western Blot (WB)

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Related Product Information for GCSF active protein

Purified recombinant GCSF protein

Product Categories/Family for GCSF active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 296011057
• NCBI GeneID: 1440
• NCBI Accession #: NP_001171618.1
• NCBI GenBank Nucleotide #: NM_001178147.1
• Molecular Weight: 19.2 kDa
• NCBI Official Full Name: granulocyte colony-stimulating factor isoform d
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cytokine; Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: positive regulation of myeloid cell differentiation; granulocyte differentiation; positive regulation of protein binding; cytokine and chemokine mediated signaling pathway; multicellular organismal development; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter