FGF1 active protein

Purified recombinant Human FGF1 protein

Cat. Num. AAA5308648

• Gene Names: FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
• Purity: > 98% pure; Purified using a proprietary chromatographic technique.
• Synonyms: FGF1; Purified recombinant Human FGF1 protein; FGF1 protein; FGF1 active protein

• Host: Human
• Purity/Purification: > 98% pure; Purified using a proprietary chromatographic technique.
• Form/Format: Supplied in lyophilized form from 1 mg/ml solution of 0.1 M NaCl, 10 mM Tris, pH 7.6
• Sequence Length: 155

• Expression: E Coli
• Bioactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 10 ng/ml, corresponding to a specific activity of > 1 x 10^5 units/mg
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Product Categories/Family for FGF1 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 380748949
• NCBI GeneID: 2246
• NCBI Accession #: NP_001244139.1
• NCBI GenBank Nucleotide #: NM_001257210.1
• UniProt Accession #: P05230; P07502; Q16588; B2R5T0; D3DQF2
• Molecular Weight: 6,698 Da
• NCBI Official Full Name: fibroblast growth factor 1 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor 1
• NCBI Official Symbol: FGF1
• NCBI Official Synonym Symbols: AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
• NCBI Protein Information: fibroblast growth factor 1
• UniProt Protein Name: Fibroblast growth factor 1
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF1
• UniProt Synonym Gene Names: FGFA; FGF-1; aFGF; ECGF; HBGF-1

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytosol; extracellular region; extracellular space; nucleoplasm

Molecular Function: 1-phosphatidylinositol-3-kinase activity; fibroblast growth factor receptor binding; growth factor activity; heparin binding; integrin binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: activation of MAPK activity; activation of protein kinase B; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; multicellular organismal development; phosphoinositide-mediated signaling; positive regulation of angiogenesis; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cholesterol biosynthetic process; positive regulation of MAP kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of phosphoinositide 3-kinase cascade