EGF active protein
EGF protein
Gene Names
EGF; URG; HOMG4
Applications
User optimized
Purity
> 98% pure
Synonyms
EGF; EGF protein; Epidermal Growth Factor protein; Urogastrone protein; URG protein; EGF active protein
Host
Human
Purity/Purification
> 98% pure
Form/Format
Supplied as a lyophilized powder. Reconstitute the lypholized rHuEGF in sterile water not less than 100ug/ml.
Sequence Length
53
Applicable Applications for EGF active protein
User optimized
Application Notes
EGF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.
Protein Type
Recombinant
Biological Significance
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
Bioactivity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is
Expression System
E Coli
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Related Product Information for EGF active protein
Purified recombinant Human EGF protein
Product Categories/Family for EGF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
6,222 Da
NCBI Official Full Name
epidermal growth factor, partial
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
EGF
NCBI Official Synonym Symbols
URG; HOMG4
NCBI Protein Information
pro-epidermal growth factor
UniProt Protein Name
Epidermal growth factor
Protein Family
UniProt Gene Name
EGF
UniProt Entry Name
Q6QBS2_HUMAN
NCBI Description
This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Research Articles on EGF
Similar Products
Product Notes
The EGF egf (Catalog #AAA538327) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EGF can be used in a range of immunoassay formats including, but not limited to, User optimized. EGF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard. Researchers should empirically determine the suitability of the EGF egf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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