CD99-L2, Fc Chimera Active Protein
CD99-L2, Fc Chimera, Recombinant, Human (CD99 antigen-like 2)
Reactivity
Human
Purity
Highly Purified95% as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
CD99-L2; Fc Chimera; Recombinant; Human (CD99 antigen-like 2); Fc Chimera active protein
Reactivity
Human
Purity/Purification
Highly Purified
95% as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
95% as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Lyophilized from a 0.2um filtered solution in PBS. Reconstitution: Add sterile PBS be added to the vial to prepare a working stock solution of 100ug/ml.
Activity
Measured by its ability to bind biotinylated rmCD99-L2 in a functional ELISA type binding assay.
Endotoxin Level
1.0 EU per 1ug of the protein as determined by the LAL method.
Preparation and Storage
Lyophilized samples are stable for up to twelve months at -20 to -70 degree C.
Upon reconstitution, this protein can be stored under sterile conditions at 2-8 degree C for one month or at -20 to -70 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Upon reconstitution, this protein can be stored under sterile conditions at 2-8 degree C for one month or at -20 to -70 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Related Product Information for CD99-L2, Fc Chimera active protein
CD99 antigen-like 2 (CD99-L2) is a 45kD type I transmembrane glycoprotein in the CD99 family of molecules (1-3). Mouse CD99-L2 cDNA encodes a 214 amino acid (aa) precursor with a 23 aa predicted signal sequence, a 116 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 54 aa cytoplasmic region (4). The ECD contains no N-linked glycosylation sites, but O-linked glycosylation is likely (1, 2). A long isoform with a 23 aa insert after aa 43 within the ECD is expressed in human, and probably in mouse (2, 5). Both long and short isoforms may have minor variants missing portions of the N-terminus of the mature protein (6). The ECD of mouse CD99-L2 short isoform shares 85%, 72% and 66% aa identity with rat, human, and bovine CD99-L2, respectively (3, 5). The mouse CD99 and CD99-L2 ECDs share only 31% aa identity, but both contain three conserved acidic motifs and are thought to originate from the same ancestral gene (1, 2). The nearly ubiquitous expression of CD99-L2 is similar to that of CD99. Mouse CD99-L2 shows highest in situ hybridization signals in neurons, cortical thymocytes, ganglia, ovarian granulosa cells, testis, and kidney, and detectable protein levels in lung, thymocytes, mouse leukocytes and vascular endothelial cells (2, 7). CD99-L2 expression on endothelial cells is reported to mediate cell aggregation and neutrophil or monocyte extravasation to inflamed tissue in vivo, while CD99 mediates lymphocyte extravasation as well (3, 7).
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Product Notes
The CD99-L2, Fc Chimera (Catalog #AAA636249) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The CD99-L2, Fc Chimera, Recombinant, Human (CD99 antigen-like 2) reacts with Human and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "CD99-L2, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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