CD117 Active Protein | CHGA active protein

CD117, Recombinant, Human (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)

Cat. Num. AAA650873

• Gene Names: CHGA; CGA
• Reactivity: Human
• Purity: 90% by SDS-PAGE.
• Synonyms: CD117; Recombinant; Human (c-kit; Stem Cell Factor; SCF Receptor; Steel Factor Receptor; Mast Cell Growth Factor; MGF); CHGA active protein

• Reactivity: Human
• Purity/Purification: 90% by SDS-PAGE.
• Form/Format: 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.

• Application Notes: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: 15 U/mg. One unit is defined as the amount of enzyme that will incorporate 1nmol phosphate to the tyrosine substrate per minute at pH 7.4 and 30 degree C.
• Assay Buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 4ug /ml recombinant c-Kit.
• Preparation and Storage: -70 degree C; Stability: >6 months at -80 degree C

Related Product Information for CHGA active protein

C-Kit is the receptor tyrosine kinase for stem cell factor (SCF) and is a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors (1,2). It plays critical controlling roles in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells (3). Upon binding with its ligand, c-Kit receptors are induced undergoing dimerization/ oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCgamma, p85 subunit of PI3 kinase, SHP2 and CrkL (4), which links c-Kit directly to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyrosine 719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

Human Kit (GenBank Accession No. BC071593), (a.a. 544-end) with N-terminal His tag,
MW 53.4kD, expressed in an Baculovirus infected Sf9 cell expression system.

Product Categories/Family for CHGA active protein

Growth Factors; Cytokines; Growth Factors-Cell Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4502805
• NCBI GeneID: 1113
• NCBI Accession #: NP_001266.1
• NCBI GenBank Nucleotide #: NM_001275.3
• UniProt Accession #: P10645; Q53FA8; Q6NR84; Q96E84; Q96GL7; Q9BQB5; B2R9E9
• Molecular Weight: 53.4kD
• NCBI Official Full Name: chromogranin-A preproprotein
• NCBI Official Synonym Full Names: chromogranin A (parathyroid secretory protein 1)
• NCBI Official Symbol: CHGA
• NCBI Official Synonym Symbols: CGA
• NCBI Protein Information: chromogranin-A; SP-I; pituitary secretory protein I; parathyroid secretory protein 1; betagranin (N-terminal fragment of chromogranin A)
• UniProt Protein Name: Chromogranin-A
• Protein Family: Chromogranin
• UniProt Gene Name: CHGA
• UniProt Synonym Gene Names: CgA; SP-I
• UniProt Entry Name: CMGA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.

Subunit structure: Interacts with SCG3

By similarity.

Subcellular location: Cytoplasmic vesicle › secretory vesicle lumen

By similarity. Cytoplasmic vesicle › secretory vesicle membrane

By similarity. Secreted. Note: Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions

By similarity.

Post-translational modification: Sulfated on tyrosine residues and/or contains sulfated glycans.O-glycosylated with core 1 or possibly core 8 glycans. Ref.16 Ref.20

Miscellaneous: Binds calcium with a low-affinity.

Sequence similarities: Belongs to the chromogranin/secretogranin protein family.