HtrA Serine Peptidase 1 Recombinant Protein | HTRA1 recombinant protein

Recombinant HtrA Serine Peptidase 1 (HTRA1)

Cat. Num. AAA2029459

• Gene Names: HTRA1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: HtrA Serine Peptidase 1; Recombinant HtrA Serine Peptidase 1 (HTRA1); HTRA1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.7 (varies by lot)
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-A LALSLGLALS PLSATAAETS SATTAQQMPS LAPMLEKVMP SVVSINVEGS TTVNTPRMPR NFQQFFGDDS PFCQEGSPFQ SSPFCQGGLGGNGGGQQQKF MALGSGVIID ADKGYVVTNN HVVDNATVIK VQLSDG
• Sequence Length: 480

• Applicable Applications for HTRA1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 15.7kDa
• Accurate Molecular Mass (KD): 15kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Escherichia coli
• Fragment: Ala10~Gly146
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 225000652
• NCBI GeneID: 5654
• Molecular Weight: 51,287 Da
• NCBI Official Full Name: HtrA serine peptidase 1
• NCBI Official Synonym Full Names: HtrA serine peptidase 1
• NCBI Official Symbol: HTRA1
• NCBI Official Synonym Symbols: L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL
• NCBI Protein Information: serine protease HTRA1
• UniProt Protein Name: Serine protease HTRA1
• Protein Family: Serine protease
• UniProt Gene Name: HTRA1
• UniProt Synonym Gene Names: HTRA; PRSS11
• UniProt Entry Name: HTRA1_HUMAN

NCBI Description

This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]

Uniprot Description

HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: extracellular matrix; extracellular space; cytosol

Molecular Function: insulin-like growth factor binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: regulation of cell growth; negative regulation of defense response to virus; proteolysis; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of epithelial cell proliferation; negative regulation of BMP signaling pathway

Disease: Macular Degeneration, Age-related, 7; Cerebral Autosomal Recessive Arteriopathy With Subcortical Infarcts And Leukoencephalopathy