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Guinea pig 5-hydroxytryptamine receptor 2B ELISA Kit | HTR2B elisa kit

Guinea pig 5-hydroxytryptamine receptor 2B (HTR2B) ELISA Kit

Reactivity
Guinea pig
Synonyms
5-hydroxytryptamine receptor 2B; Guinea pig 5-hydroxytryptamine receptor 2B (HTR2B) ELISA Kit; 5-HT(2B); 5-HT2B; 5-HT 2B receptor|5-hydroxytryptamine 2B receptor; HTR2B elisa kit
Ordering
For Research Use Only!
Reactivity
Guinea pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Guinea pig HTR2B. No significant cross-reactivity or interference between Guinea pig HTR2B and analogues was observed.
Sequence Length
481
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for HTR2B elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HTR2B in samples. An antibody specific for HTR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHTR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
11,226 Da
NCBI Official Full Name
5-hydroxytryptamine receptor 2B
UniProt Protein Name
5-hydroxytryptamine receptor 2B
UniProt Gene Name
HTR2B
UniProt Synonym Gene Names
5-HT-2B; 5-HT2B

Uniprot Description

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. May play a role in the perception of pain. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine ().

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Product Notes

The Guinea pig HTR2B htr2b (Catalog #AAA284333) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284333 ELISA Kit recognizes Guinea pig HTR2B. It is sometimes possible for the material contained within the vial of "5-hydroxytryptamine receptor 2B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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