HSP70B recombinant protein

HSP70B, 1-643aa, Human, His tag, E Coli

Cat. Num. AAA203423

• Gene Names: HSPA6; HSP70B'
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: HSP70B; 1-643aa; Human; His tag; E Coli; Heat shock 70 kDa protein 6; HSPA6'; Heat shock 70 kDa protein 6 Heat shock 70 kDa protein B'; ; HSP70B recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 100mM NaCl, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MQAPRELAVG IDLGTTYSCV GVFQQGRVEI LANDQGNRTT PSYVAFTDTE RLVGDAAKSQ AALNPHNTVF DAKRLIGRKF ADTTVQSDMK HWPFRVVSEG GKPKVRVCYR GEDKTFYPEE ISSMVLSKMK ETAEAYLGQP VKHAVITVPA YFNDSQRQAT KDAGAIAGLN VLRIINEPTA AAIAYGLDRR GAGERNVLIF DLGGGTFDVS VLSIDAGVFE VKATAGDTHL GGEDFDNRLV NHFMEEFRRK HGKDLSGNKR ALRRLRTACE RAKRTLSSST QATLEIDSLF EGVDFYTSIT RARFEELCSD LFRSTLEPVE KALRDAKLDK AQIHDVVLVG GSTRIPKVQK LLQDFFNGKE LNKSINPDEA VAYGAAVQAA VLMGDKCEKV QDLLLLDVAP LSLGLETAGG VMTTLIQRNA TIPTKQTQTF TTYSDNQPGV FIQVYEGERA MTKDNNLLGR FELSGIPPAP RGVPQIEVTF DIDANGILSV TATDRSTGKA NKITITNDKG RLSKEEVERM VHEAEQYKAE DEAQRDRVAA KNSLEAHVFH VKGSLQEESL RDKIPEEDRR KMQDKCREVL AWLEHNQLAE KEEYEHQKRE LEQICRPIFS RLYGGPGVPG GSSCGTQARQ GDPSTGPIIE EVD
• Sequence Length: 643

• Applicable Applications for HSP70B recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HSP70B recombinant protein

HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for HSP70B recombinant protein

Heat Shock Proteins

References

Noonan E., et al. (2008). Exp Cell Res. 314 (13):2468-76.; Hightower LE., et al. (2007). Cell Stress Chaperones. 12 (4):393-402.;

NCBI and Uniprot Product Information

• NCBI GI #: 34419635
• NCBI GeneID: 3310
• NCBI Accession #: NP_002146
• NCBI GenBank Nucleotide #: NM_002155.4
• UniProt Accession #: P17066; Q1HBA8; Q8IYK7; Q9BT95
• Molecular Weight: 73.2 kDa (663aa)
• NCBI Official Full Name: heat shock 70 kDa protein 6
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 6
• NCBI Official Symbol: HSPA6
• NCBI Official Synonym Symbols: HSP70B'
• NCBI Protein Information: heat shock 70 kDa protein 6
• UniProt Protein Name: Heat shock 70 kDa protein 6
• UniProt Gene Name: HSPA6
• UniProt Synonym Gene Names: HSP70B'

Uniprot Description

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365).