Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) Recombinant Protein | HSPA1L recombinant protein

Recombinant Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)

Cat. Num. AAA2009342

• Gene Names: HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Heat Shock 70kDa Protein 1 Like Protein (HSPA1L); Recombinant Heat Shock 70kDa Protein 1 Like Protein (HSPA1L); HSPA1L recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MATAKGIAIG IDLGTTYSCV GVFQHGKVEI IANDQGNRTT PSYVAFTDTE RLIGDAAKNQ VAMNPQNTVF DAKRLIGRKF NDPVVQADMK LWPFQVINEG GKPKVLVSYK GENKAFYPEE ISSMVLTKLK ETAEAFLGHP VTNAVITVPA YFNDSQRQAT KDAGVIAGLN VLRIINEPTA AAIAYGLDKG GQGERHVLIF DLGGGTFDVS ILTIDDGIFE VKATAGDTHL GGEDFDNRLV SHFVEEFKRK HKKDISQNKR AVRRLRTACE RAKRTLSSST QANLEIDSLY EGIDFYTSIT RARFEELCAD LFRGTLEPVE KALRDAKMDK AKIHDIVLVG GSTRIPKVQR LLQDYFNGRD LNKSINPDEA VAYGAAVQAA ILMGDKSEKV QDLLLLDVAP LSLGLETAGG VMTALIKRNS TIPTKQTQIF TTYSDNQPGV LIQVYEGERA MTKDNNLLGR FDLTGIPPAP RGVPQIEVTF DIDANGILNV TATDKSTGKV NKITITNDKG RLSKEEIERM VLDAEKYKAE DEVQREKIAA KNALESYAFN MKSVVSDEGL KGKISESDKN KILDKCNELL SWLEVNQLAE KDEFDHKRKE LEQMCNPIIT KLYQGGCTGP ACGTGYVPGR PATGPTIEEV D
• Sequence Length: 641

• Applicable Applications for HSPA1L recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Asp641 (Accession # P34931) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 124256496
• NCBI GeneID: 3305
• NCBI Accession #: NP_005518.3
• NCBI GenBank Nucleotide #: NM_005527.3
• UniProt Accession #: P34931; O75634; Q2HXR3; Q8NE72; Q96QC9; Q9UQM1; A6NNB0; B0UXW8
• Molecular Weight: 71.9kDa
• NCBI Official Full Name: heat shock 70 kDa protein 1-like
• NCBI Official Synonym Full Names: heat shock 70kDa protein 1-like
• NCBI Official Symbol: HSPA1L
• NCBI Official Synonym Symbols: HSP70T; hum70t; HSP70-1L; HSP70-HOM
• NCBI Protein Information: heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1L; heat shock 70kD protein-like 1; heat shock 10kDa protein 1-like; heat shock 70 kDa protein 1-Hom
• UniProt Protein Name: Heat shock 70 kDa protein 1-like
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: HSPA1L
• UniProt Synonym Gene Names: Heat shock 70 kDa protein 1L; HSP70-Hom
• UniProt Entry Name: HS71L_HUMAN

NCBI Description

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008]

Uniprot Description

HSPA1L: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Not induced by heat shock. Expressed in spermatids. Belongs to the heat shock protein 70 family.

Protein type: Mitochondrial; Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; signalosome; mitochondrial matrix; cytosol

Molecular Function: protein binding; unfolded protein binding; ATP binding

Biological Process: binding of sperm to zona pellucida; protein refolding; response to unfolded protein