Heat Shock Protein 27 Recombinant Protein | Hsp27 recombinant protein
Heat Shock Protein 27, Recombinant, Human (HSP27)
Gene Names
Hsp27; 27; 27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; DmHSP27; hsp 27; Hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
Applications
ELISA, Western Blot
Purity
Highly Purified90% pure as determined by SDS-PAGE analysis.
Synonyms
Heat Shock Protein 27; Recombinant; Human (HSP27); Hsp27 recombinant protein
Purity/Purification
Highly Purified
90% pure as determined by SDS-PAGE analysis.
90% pure as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 20mM phosphate buffer, 150mM sodium chloride and 10% glycerol.
Applicable Applications for Hsp27 recombinant protein
ELISA (EL/EIA), Western Blot (WB)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Hsp27 recombinant protein
Hsp27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an a-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The upregulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (6).
Product Categories/Family for Hsp27 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,617 Da
NCBI Official Full Name
heat shock protein 27
NCBI Official Synonym Full Names
Heat shock protein 27
NCBI Official Symbol
Hsp27
NCBI Official Synonym Symbols
27; 27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; DmHSP27; hsp 27; Hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
NCBI Protein Information
CG4466 gene product from transcript CG4466-RA; CG4466-PA; Hsp 27; Hsp27-PA; heat shock protein 237; heat shock protein 27; heat shock protein hsp27
UniProt Protein Name
Heat shock protein 27
Protein Family
UniProt Gene Name
Hsp27
UniProt Entry Name
HSP27_DROME
Uniprot Description
Sequence similarities: Belongs to the small heat shock protein (HSP20) family.
Research Articles on Hsp27
Similar Products
Product Notes
The Hsp27 hsp27 (Catalog #AAA636825) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock Protein 27 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Researchers should empirically determine the suitability of the Hsp27 hsp27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 27, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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