Heparanase (HPA) Recombinant Protein | HPA recombinant protein

Recombinant Heparanase (HPA)

Cat. Num. AAA2011723

• Gene Names: Hpse; Hep; Hpa
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Heparanase (HPA); Recombinant Heparanase (HPA); HPA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS- R LFQSVSPSFL SITIDASLAT DPRFLTFLGS PRLRALARGL SPAYLRFGGT KTDFLIFDPN KEPTSEERSY WQSQDNNDIC GSERVSADVL RKLQMEWPFQ ELLLLREQYQ REFKNSTYSR SSVDMLYSFA KCSRLDLIFG LNALLRTP
• Sequence Length: 536

• Applicable Applications for HPA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Arg40~Pro188 (Accession # Q71RP1) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 12018276
• NCBI GeneID: 64537
• NCBI Accession #: NP_072127.1
• NCBI GenBank Nucleotide #: NM_022605.1
• UniProt Accession #: Q71RP1; Q9QZF8
• Molecular Weight: 18.4kDa
• NCBI Official Full Name: heparanase
• NCBI Official Synonym Full Names: heparanase
• NCBI Official Symbol: Hpse
• NCBI Official Synonym Symbols: Hep; Hpa
• NCBI Protein Information: heparanase; heparanase-like; endo-glucoronidase
• UniProt Protein Name: Heparanase
• UniProt Gene Name: Hpse
• UniProt Synonym Gene Names: Hep
• UniProt Entry Name: HPSE_RAT

NCBI Description

endo-beta-glucuronidase capable of cleaving heparan sulfate involved in the regulation of heparin sulfate proteoglycan function [RGD, Feb 2006]

Uniprot Description

Function: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis

By similarity. Ref.3

Catalytic activity: Endohydrolysis of (1->4)-beta-D-glycosidic bonds of heparan sulfate chains in heparan sulfate proteoglycan.

Enzyme regulation: Inhibited by laminarin sulfate and, to a lower extent, by heparin and sulfamin

By similarity. Activated by calcium and magnesium. Inhibited by EDTA. Ref.2

Subunit structure: Heterodimer; heterodimer formation between the 8 kDa and the 50 kDa subunits is required for enzyme activity

By similarity. Interacts with TF; the interaction, inhibited by heparin, enhances the generation of activated factor X and activates coagulation. Interacts with HRG; the interaction is enhanced at acidic pH, partially inhibits binding of HPSE to cell surface receptors and modulates its enzymatic activity. Interacts with SDC1; the interaction enhances the shedding of SDC1. Interacts with HPSE2

By similarity.

Subcellular location: Lysosome membrane; Peripheral membrane protein

By similarity. Secreted

By similarity. Nucleus

By similarity. Note: Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium. Associates with lipid rafts

By similarity.

Post-translational modification: Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and the 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme

By similarity.N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility

By similarity.

Sequence similarities: Belongs to the glycosyl hydrolase 79 family.