HP recombinant protein

HP, 19-347aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205696

• Gene Names: HP; BP; HPA1S; HP2ALPHA2
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: HP; 19-347aa; Human; His tag; E Coli (Denatured); Haptoglobin isoform 2 preproprotein; BP; HP2ALPHA2; HPA1S; HP recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.4M urea
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSVDSGNDV TDIADDGCPK PPEIAHGYVE HSVRYQCKNY YKLRTEGDGV YTLNNEKQWI NKAVGDKLPE CEAVCGKPKN PANPVQRILG GHLDAKGSFP WQAKMVSHHN LTTGATLINE QWLLTTAKNL FLNHSENATA KDIAPTLTLY VGKKQLVEIE KVVLHPNYSQ VDIGLIKLKQ KVSVNERVMP ICLPSKDYAE VGRVGYVSGW GRNANFKFTD HLKYVMLPVA DQDQCIRHYE GSTVPEKKTP KSPVGVQPIL NEHTFCAGMS KYQEDTCYGD AGSAFAVHDL EEDTWYATGI LSFDKSCAVA EYGVYVKVTS IQDWVQKTIA EN
• Sequence Length: 347

• Applicable Applications for HP recombinant protein: SDS-PAGE (SDS), Denatured
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for HP recombinant protein

HP, as known as haptoglobin, is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen). Recombinant human HP protein, fused to His-tag at N-terminus, was expressed in E Coli

Product Categories/Family for HP recombinant protein

Cardiovascular

References

Dobryszycka W. et al. (1997) Eur J Clin Chem Clin Biochem. 35:647-654.; Wassell J. et al. (2000) Clin. Lab. 46:547-552.;

NCBI and Uniprot Product Information

• NCBI GI #: 186910296
• NCBI GeneID: 3240
• NCBI Accession #: NP_001119574
• NCBI GenBank Nucleotide #: NM_001126102.2
• UniProt Accession #: P00738; P00737; Q0VAC4; Q0VAC5; Q2PP15; Q3B7J0; Q6LBY9; Q9UC67; B0AZL5
• Molecular Weight: 39.0 kDa (352aa)
• NCBI Official Full Name: haptoglobin isoform 2 preproprotein
• NCBI Official Synonym Full Names: haptoglobin
• NCBI Official Symbol: HP
• NCBI Official Synonym Symbols: BP; HPA1S; HP2ALPHA2
• NCBI Protein Information: haptoglobin
• UniProt Protein Name: Haptoglobin
• Protein Family: HPr-like protein
• UniProt Gene Name: HP

NCBI Description

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.