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Typical Testing Data/Standard Curve (for reference only) ()

Canine Heme oxygenase 2 ELISA Kit | HO-2 elisa kit

Canine heme oxygenase 2 (HO-2) ELISA Kit

Gene Names
Hmox2; HO2; HO-2
Reactivity
Canine
Synonyms
Heme oxygenase 2; Canine heme oxygenase 2 (HO-2) ELISA Kit; HO-2 elisa kit
Ordering
For Research Use Only!
Reactivity
Canine
Specificity
No cross-reaction with other factors.
Sequence Length
315
Samples
Canine serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
10 ng/mL-0.156 ng/mL
Sensitivity
Up to 0.05 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for HO-2 elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Canine HO-2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,739 Da
NCBI Official Full Name
heme oxygenase 2
NCBI Official Synonym Full Names
heme oxygenase 2
NCBI Official Symbol
Hmox2
NCBI Official Synonym Symbols
HO2; HO-2
NCBI Protein Information
heme oxygenase 2
UniProt Protein Name
Heme oxygenase 2
Protein Family
UniProt Gene Name
Hmox2
UniProt Synonym Gene Names
HO-2
UniProt Entry Name
HMOX2_MOUSE

Uniprot Description

HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.

Protein type: Oxidoreductase; EC 1.14.99.3; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; membrane; plasma membrane

Molecular Function: heme oxygenase (decyclizing) activity; metal ion binding; oxidoreductase activity

Biological Process: heme oxidation; response to hypoxia; response to oxidative stress

Research Articles on HO-2

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Product Notes

The Canine HO-2 hmox2 (Catalog #AAA2604973) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2604973 ELISA Kit recognizes Canine HO-2. It is sometimes possible for the material contained within the vial of "Heme oxygenase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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