Human Heterogeneous nuclear ribonucleoprotein K ELISA Kit | HNRNPK elisa kit

Human Heterogeneous nuclear ribonucleoprotein K ELISA Kit

Cat. Num. AAA764501

• Gene Names: Hnrnpk; Csbp; Hnrpk
• Reactivity: Human
• Synonyms: Heterogeneous nuclear ribonucleoprotein K; Human Heterogeneous nuclear ribonucleoprotein K ELISA Kit; HNRNPK/HNRPK/Heterogeneous nuclear ribonucleoprotein K/Transformation up-regulated nuclear protein/TUNP; HNRNPK elisa kit

• Reactivity: Human
• Sequence Length: 463

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 39-2500pg/ml
• Sensitivity: 22.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HNRNPK elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- HNRNPK antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- HNRNPK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HNRNPK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HNRNPK can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16923998
• NCBI GeneID: 117282
• NCBI Accession #: NP_476482.1
• NCBI GenBank Nucleotide #: NM_057141.1
• Molecular Weight: 50,976 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein K
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein K
• NCBI Official Symbol: Hnrnpk
• NCBI Official Synonym Symbols: Csbp; Hnrpk
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein K
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein K
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: Hnrnpk
• UniProt Synonym Gene Names: Hnrpk; hnRNP K; CSBP
• UniProt Entry Name: HNRPK_RAT

NCBI Description

DNA and pre-mRNA binding protein, binds primarily with cytidine-rich (poly-C) sequences [RGD, Feb 2006]

Uniprot Description

hnRNP K: a heterogeneous nuclear ribonucleoprotein (hnRNP). One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Spliceosome; RNA splicing

Cellular Component: cell cortex; cell junction; cytosol; dendritic spine; focal adhesion; membrane; mitochondrion; nerve terminal; nuclear chromatin; nucleoplasm; nucleus; podosome; postsynaptic density; spliceosome

Molecular Function: actinin binding; ATPase binding; double-stranded DNA binding; heat shock protein binding; lamin binding; mRNA 3'-UTR binding; pre-mRNA 3'-splice site binding; protein binding; protein domain specific binding; ribonucleoprotein binding; RNA binding; single-stranded DNA binding; transcription factor binding

Biological Process: acute-phase response; aging; camera-type eye development; cellular response to insulin stimulus; central nervous system development; cerebellum development; cerebral cortex development; hippocampus development; kidney development; liver development; lung development; mRNA processing; negative regulation of apoptosis; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; ovarian follicle development; peripheral nervous system development; positive regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of receptor-mediated endocytosis; positive regulation of RNA splicing; positive regulation of synaptic transmission; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; response to activity; RNA splicing; thymus development; transcription from RNA polymerase II promoter; transcription, DNA-dependent