HO2 recombinant protein

HO2 recombinant protein

Cat. Num. AAA515096

• Gene Names: HMOX2; HO-2
• Applications: Western Blot
• Synonyms: HO2; HO2 recombinant protein; HMOX2

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 1754

• Applicable Applications for HO2 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human HO2 Protein was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for HO2 recombinant protein

Recombinant full-length human HO2 Protein was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: HO2, or heme oxygenase 2, belongs to the heme oxygenase family that are essential enzyme in heme catabolism that cleave heme to form biliverdin which is subsequently converted to bilirubin by biliverdin reductase (1). HO2 is part of the calcium-sensitive potassium channel complex and enhances channel activity in normoxia (2). Knockdown of HO2 expression reduces channel activity, an effect that is reversed by carbon monoxide. Inhibition of calcium-sensitive potassium channels by hypoxia is dependent on HO2 expression and is augmented by HO2 stimulation.

Product Categories/Family for HO2 recombinant protein

Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins

References

1.Kemp, P.J. et al: Hemeoxygenase-2 as an O2 sensor in K+ channel-dependent chemotransduction. Biochem Biophys Res Commun. 2005;338(1):648-52.
2.Williams, S. E. et al: Hemoxygenase-2 is an oxygen sensor for a calcium-sensitive potassium channel. Science. 2004 Dec 17;306(5704):2093-7.

NCBI and Uniprot Product Information

• NCBI GI #: 187761305
• NCBI GeneID: 3163
• NCBI Accession #: NP_002125.3
• NCBI GenBank Nucleotide #: NM_002134
• UniProt Accession #: P30519; O60605; A8MT35; D3DUD5
• Molecular Weight: ~62kDa
• NCBI Official Full Name: Homo sapiens heme oxygenase (decycling) 2 (HMOX2), transcript variant 3, mRNA
• NCBI Official Synonym Full Names: heme oxygenase (decycling) 2
• NCBI Official Symbol: HMOX2
• NCBI Official Synonym Symbols: HO-2
• NCBI Protein Information: heme oxygenase 2
• UniProt Protein Name: Heme oxygenase 2
• Protein Family: Probable inactive heme oxygenase
• UniProt Gene Name: HMOX2
• UniProt Synonym Gene Names: HO2; HO-2
• UniProt Entry Name: HMOX2_HUMAN

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Catalytic activity: Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

Subcellular location: Microsome. Endoplasmic reticulum.

Induction: Heme oxygenase 2 activity is non-inducible.

Sequence similarities: Belongs to the heme oxygenase family.Contains 2 HRM (heme regulatory motif) repeats.