Heme oxygenase1 Recombinant Protein | HMOX1 recombinant protein

Heme oxygenase1,1-266aa, Human, His tag, E Coli

Cat. Num. AAA203285

• Gene Names: HMOX1; HO-1; HSP32; HMOX1D; bK286B10
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Heme oxygenase1; 1-266aa; Human; His tag; E Coli; HO-1; Heat shock protein 32; HSP32; bK286B10; D8Wsu38e; Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; Hmox; HMOX 1; HMOX1; HO; HO 1; HO1.; HMOX1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 50mM NaCl, 0.1mM PMSF, 10% glycerol
• Concentration: 1 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MERPQPHSMP QDLSEALKEA TKEVHTQAEN AEFMRNFQKG QVTRDGFKLV MASLYHIYVA LEEEIERNKE SPVFAPVYFP EELHRKAALE QDLAFWYGPR WQEVIPYTPA MQRYVKRLHE VGRTEPELLV AHAYTRYLGD LSGGQVLKKI AQKALDLPSS GEGLAFFTFP NIASATKFKQ LYRSRMNSLE MTPAVRQRVI EEAKTAFLLN IQLFEELQEL LTHDTKDQSP SRAPGLRQRA SNKVQDSAPV ETPRGKPPLN TRSQAPLEHH HHHH
• Sequence Length: 288

• Applicable Applications for HMOX1 recombinant protein: SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks).; For long term storage, aliquot and store at -20°C or -70°C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HMOX1 recombinant protein

Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Also this protein is known to play an important role in the regulation of cardiovascular function and its adaptive response to a variety of stressors. Recombinant human Heme oxygenase 1 protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for HMOX1 recombinant protein

Redox Proteins

References

Vareille M., et al. (2008). J Immunol. 180 (8):5720-6.; Soares MP., et al. (2001). Immunol Rev.184:275-85;

NCBI and Uniprot Product Information

• NCBI GI #: 4504437
• NCBI GeneID: 3162
• NCBI Accession #: NP_002124
• NCBI GenBank Nucleotide #: NM_002133.2
• UniProt Accession #: P09601
• Molecular Weight: 31.4 kDa (274 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: heme oxygenase 1
• NCBI Official Synonym Full Names: heme oxygenase 1
• NCBI Official Symbol: HMOX1
• NCBI Official Synonym Symbols: HO-1; HSP32; HMOX1D; bK286B10
• NCBI Protein Information: heme oxygenase 1
• UniProt Protein Name: Heme oxygenase 1
• UniProt Gene Name: HMOX1
• UniProt Synonym Gene Names: HO; HO1; HO-1

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]

Uniprot Description

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.