Human Non-histone chromosomal protein HMG-14 ELISA Kit | HMGN1 elisa kit

Human Non-histone chromosomal protein HMG-14 ELISA Kit

Cat. Num. AAA760202

• Gene Names: HMGN1; HMG14
• Reactivity: Human
• Synonyms: Non-histone chromosomal protein HMG-14; Human Non-histone chromosomal protein HMG-14 ELISA Kit; HMGN1/HMG14/High mobility group nucleosome-binding domain-containing protein 1/high-mobility group (nonhistone chromosomal) protein 14/high-mobility group nucleosome binding 1/high-mobility group nucleosome binding domain 1//non-histone chromosomal protein HMG-14/nonhistone chromosomal protein HMG-14; HMGN1 elisa kit

• Reactivity: Human
• Sequence Length: 100

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HMGN1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human HMGN1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HMGN1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HMGN1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HMGN1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 48255933
• NCBI GeneID: 3150
• NCBI Accession #: NP_004956.5
• NCBI GenBank Nucleotide #: NM_004965.6
• Molecular Weight: 10,659 Da
• NCBI Official Full Name: non-histone chromosomal protein HMG-14
• NCBI Official Synonym Full Names: high mobility group nucleosome binding domain 1
• NCBI Official Symbol: HMGN1
• NCBI Official Synonym Symbols: HMG14
• NCBI Protein Information: non-histone chromosomal protein HMG-14
• UniProt Protein Name: Non-histone chromosomal protein HMG-14
• Protein Family: Non-histone chromosomal protein
• UniProt Gene Name: HMGN1
• UniProt Synonym Gene Names: HMG14
• UniProt Entry Name: HMGN1_HUMAN

NCBI Description

The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011]

Uniprot Description

HMGN1: a nonhistone chromosomal protein of the HMG-14/HMG-17 protein family. Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation.

Protein type: Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: chromatin; cytoplasm; nucleoplasm; nucleus

Molecular Function: DNA binding; nucleosomal DNA binding

Biological Process: DNA repair; establishment and/or maintenance of chromatin architecture; nucleotide-excision repair; positive regulation of RNA elongation; post-embryonic camera-type eye morphogenesis; pyrimidine dimer repair via nucleotide-excision repair; regulation of development, heterochronic; regulation of epithelial cell proliferation; regulation of transcription from RNA polymerase II promoter; response to UV-B; response to UV-C; transcription-coupled nucleotide-excision repair