High Mobility Group AT Hook Protein 1 (HMGA1) Recombinant Protein | HMGA1 recombinant protein

Recombinant High Mobility Group AT Hook Protein 1 (HMGA1)

Cat. Num. AAA2010911

• Gene Names: Hmga1; Hmgi; Hmgy; Hmgiy; Hmga1a; Hmga1b; AL023995
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: High Mobility Group AT Hook Protein 1 (HMGA1); Recombinant High Mobility Group AT Hook Protein 1 (HMGA1); HMGA1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MSESGSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE VPTPKRPRGR PKGSKNKGAA KTRKVTTAPG RKPRGRPKKL EKEEEEGISQ ESSEEEQ
• Sequence Length: 107

• Applicable Applications for HMGA1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Gln107 (Accession # P17095) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 70909308
• NCBI GeneID: 15361
• NCBI Accession #: NP_001020598.1
• NCBI GenBank Nucleotide #: NM_001025427.3
• UniProt Accession #: P17095; Q91WV2; Q924L7; Q924L8
• Molecular Weight: 13.1kDa
• NCBI Official Full Name: high mobility group protein HMG-I/HMG-Y isoform a
• NCBI Official Synonym Full Names: high mobility group AT-hook 1
• NCBI Official Symbol: Hmga1
• NCBI Official Synonym Symbols: Hmgi; Hmgy; Hmgiy; Hmga1a; Hmga1b; AL023995
• NCBI Protein Information: high mobility group protein HMG-I/HMG-Y; high mobility group protein I; high mobility group protein A1; high mobility group AT-hook protein 1
• UniProt Protein Name: High mobility group protein HMG-I/HMG-Y
• Protein Family: High mobility group protein
• UniProt Gene Name: Hmga1
• UniProt Synonym Gene Names: Hmgi; Hmgiy; HMG-I(Y)
• UniProt Entry Name: HMGA1_MOUSE

NCBI Description

This locus encodes a member of the nuclear, non-histone high mobility group protein family. This architectural transcription factor binds to A-T rich DNA sequences and participates in enhanceosome formation, chromatin remodeling and regulation of transcription. This protein functions in many cellular processes, including cell growth and differentiation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Uniprot Description

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: focal adhesion; nucleus

Molecular Function: retinoid X receptor binding; peroxisome proliferator activated receptor binding; enzyme binding; DNA-(apurinic or apyrimidinic site) lyase activity; ligand-dependent nuclear receptor transcription coactivator activity; retinoic acid receptor binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; base-excision repair; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; DNA catabolic process, endonucleolytic