Major Histocompatibility Complex Class II DR Alpha Recombinant Protein | HLA-DRA recombinant protein

Recombinant Human Major Histocompatibility Complex Class II DR Alpha

Cat. Num. AAA146342

• Gene Names: HLA-DRA; MLRW; HLA-DRA1
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Major Histocompatibility Complex Class II DR Alpha; Recombinant Human Major Histocompatibility Complex Class II DR Alpha; HLA-DRA Human; Major Histocompatibility Complex Class II DR Alpha Human Recombinant; Major Histocompatibility Complex; Class II; DR Alpha; HLA-DRA1; MHC Class II AntigenDRA; MLRW; Histocompatibility AntigenHLA-DR Alpha; HLAClass II Histocompatibility Antigen; DR Alpha Chain; MHC Cell Surface Glycoprotein; HLA-DRA recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: HLA-DRA protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MIKEEHVIIQ AEFYLNPDQS GEFMFDFDGD EIFHVDMAKK ETVWRLEEFG RFASFEAQGALANIAVDKAN LEIMTKRSNY TPITNVPPEV TVLTNSPVEL REPNVLICFI DKFTPPVVNV TWLRNGKPVTTGVSETVFLP REDHLFRKFH YLPFLPSTED VYDCRVEHWG LDEPLLKHWE FDAPSPLPET TE
• Sequence Length: 254

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

Related Product Information for HLA-DRA recombinant protein

Description: HLA-DRA Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 212 amino acids (26-216 a.a) and having a molecular mass of 24.3kDa.HLA-DRA is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Major histocompatibility complex, class II, DR alpha (HLA-DRA) belongs to the MHC class II family. HLA-DRA binds peptides derived from antigens which access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for identification by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mainly by degradation of proteins which access the endocytic route, where they are processed by lysosomal proteases and other hydrolases.

Product Categories/Family for HLA-DRA recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Major Histocompatibility Complex

NCBI and Uniprot Product Information

• NCBI GI #: 52426774
• NCBI GeneID: 3122
• NCBI Accession #: NP_061984.2
• NCBI GenBank Nucleotide #: NM_019111.4
• UniProt Accession #: P01903; Q30160; Q6IAZ1; Q861I2; Q9TP70; A2BET4
• Molecular Weight: 28,607 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DR alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DR alpha
• NCBI Official Symbol: HLA-DRA
• NCBI Official Synonym Symbols: MLRW; HLA-DRA1
• NCBI Protein Information: HLA class II histocompatibility antigen, DR alpha chain; MHC cell surface glycoprotein; MHC class II antigen DRA; histocompatibility antigen HLA-DR alpha
• UniProt Protein Name: HLA class II histocompatibility antigen, DR alpha chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DRA
• UniProt Synonym Gene Names: HLA-DRA1
• UniProt Entry Name: DRA_HUMAN

NCBI Description

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DRA: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; cell surface; integral to plasma membrane; lysosome; late endosome membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: peptide antigen assembly with MHC class II protein complex; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; cognition; T cell receptor signaling pathway; antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; polysaccharide assembly with MHC class II protein complex

Disease: Hepatitis B Virus, Susceptibility To