SDS-Page
(Recombinant Histone H4 trimethyl Lys5 tested by SDS-PAGE gel. SDS-PAGE analysis of 5 ug Recombinant Histone H4 monomethyl Lys5 (lane 2), Recombinant Histone H4 dimethyl Lys5 (lane 3), and Recombinant Histone H4 trimethyl Lys5 (lane 4). Molecular weight marker is in lane 1.)
Histone H4K5me3 Recombinant Protein | H4K5me3 recombinant protein
Recombinant Histone H4K5me3 (MLA)
Purity
The recombinant histone is >98% pure by SDS-PAGE.
Synonyms
Histone H4K5me3; Recombinant Histone H4K5me3 (MLA); H4K5me3 recombinant protein
Host
E Coli
Purity/Purification
The recombinant histone is >98% pure by SDS-PAGE.
Form/Format
50ug supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperat
Protein Species
Xenopus
Conjugation
Methylated
Notes
Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Lyophilized proteins can be stored at -20 degree C or -80 degree C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant Histone H4 trimethyl Lys5 tested by SDS-PAGE gel. SDS-PAGE analysis of 5 ug Recombinant Histone H4 monomethyl Lys5 (lane 2), Recombinant Histone H4 dimethyl Lys5 (lane 3), and Recombinant Histone H4 trimethyl Lys5 (lane 4). Molecular weight marker is in lane 1.)
SDS-Page
(Recombinant Histone H4 trimethyl Lys5 tested by SDS-PAGE gel. SDS-PAGE analysis of 5 ug Recombinant Histone H4 monomethyl Lys5 (lane 2), Recombinant Histone H4 dimethyl Lys5 (lane 3), and Recombinant Histone H4 trimethyl Lys5 (lane 4). Molecular weight marker is in lane 1.)
Related Product Information for H4K5me3 recombinant protein
Short Description: Recombinant Xenopus laevis Histone H4 trimethyl Lys5 (H4K5me3) is produced in E Coli and purified using FPLC. Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). This specific chemical treatment enables the site and degree of methylation to be controlled precisely. Each methylation reaction is over 99% complete, as verified by high-resolution ESI-TOF mass spectrometry. Protein concentration was determined using the molar extinction coefficient for Histone H4 and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE. The molecular weight of the recombinant histone is 11,297 Daltons. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Recombinant histones are used to analyze histone modifications, or to generate chromatin in vitro.
Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.
Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.
Product Categories/Family for H4K5me3 recombinant protein
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Product Notes
The H4K5me3 (Catalog #AAA388055) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Histone H4K5me3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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