Histone H4K20me3 Recombinant Protein | H4K20me3 recombinant protein

Recombinant Histone H4K20me3 (MLA), His-Tag

Cat. Num. AAA388323

• Gene Names: HIST2H4A; H4; H4/n; H4F2; H4FN; FO108; HIST2H4
• Synonyms: Histone H4K20me3; Recombinant Histone H4K20me3 (MLA); His-Tag; H4K20me3 recombinant protein

• Host: E Coli
• Form/Format: Recombinant Histone H4K20me3 (MLA), His-Tag is supplied in 25mM Tris-HCl pH7.4, 150mM NaCl and 10% glycerol.

• Protein Species: Human
• Tag: His-Tag
• Conjugation: Methylated
• Notes: Recombinant Histone H4K20me3 (MLA), His-Tag is suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant Histone H4K20me3 (MLA), His-Tag protein gel 13% SDS-PAGE gel, stained with Coomassie Blue. MW: 12.4 kDa Purity: > 90%)

Testing Data

(ESI-TOF Mass Spec analysis for Recombinant Histone H4K20me3 (MLA), His-Tag Expected mass: 12362 Da Observed mass: 12362 Da)

Related Product Information for H4K20me3 recombinant protein

Short Description: Recombinant Histone H4K20me3 (MLA), His-Tag was expressed in E Coli as full-length protein (accession number NP_003539.1) with a C-terminal 6xHis-tag, of which the Lysine 20 was mutated to Cysteine and then tri-methylated via MLA technology. The molecular weight of this protein is 12362 Da. Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

Background: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/ exit points. Recombinant Histone H4K20me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and c?-provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

Product Categories/Family for H4K20me3 recombinant protein

Recombinant Proteins; Histone

NCBI and Uniprot Product Information

• NCBI GI #: 4504323
• NCBI GeneID: 8370
• NCBI Accession #: NP_003539.1
• NCBI GenBank Nucleotide #: NM_003548.2
• UniProt Accession #: P62805; P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0
• Molecular Weight: 103
• NCBI Official Full Name: histone H4
• NCBI Official Synonym Full Names: histone cluster 2, H4a
• NCBI Official Symbol: HIST2H4A
• NCBI Official Synonym Symbols: H4; H4/n; H4F2; H4FN; FO108; HIST2H4
• NCBI Protein Information: histone H4; histone 2, H4a; H4 histone, family 2; histone IV, family 2; H4 histone family, member N
• UniProt Protein Name: Histone H4
• UniProt Gene Name: HIST1H4A
• UniProt Synonym Gene Names: H4/A; H4FA
• UniProt Entry Name: H4_HUMAN

Uniprot Description

Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular location: Nucleus. Chromosome.

Post-translational modification: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ref.19 Ref.20 Ref.21 Ref.23 Ref.24 Ref.28Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ref.28 Ref.36Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ref.27 Ref.30Sumoylated, which is associated with transcriptional repression. Ref.22Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Ref.35

Sequence similarities: Belongs to the histone H4 family.

Sequence caution: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.