HIST1H3A recombinant protein

HIST1H3A Protein, Human, Mouse, Recombinant

Cat. Num. AAA8120753

• Gene Names: HIST1H3A; H3/A; H3FA
• Purity: >95% as determined by SDS-PAGE
• Synonyms: HIST1H3A; HIST1H3A Protein; Human; Mouse; Recombinant; Human/Mouse Histone H3.1/HIST1H3A/H3FA Protein; histone cluster 1; H3a; HIST1H3A recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 2mM beta-Mercaptoethanol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala136

• Species: Human, Mouse
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the native human/mouse HIST1H3A (NP_003520.1)(Met1-Ala136) was expressed. Human and Mouse HIST1H3A sequences are identical.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HIST1H3A recombinant protein

Background: Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is expressed during the S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures.

Product Categories/Family for HIST1H3A recombinant protein

MAPK-Erk Pathway

References

Lachner M, et al., 2001, Nature 410 (6824): 116-20. Koessler H, et al., 2003, DNA Cell Biol. 22 (4): 233-41. Macdonald N. et al., 2005, Mol. Cell 20: 199-211. Hyllus D. et al., 2007, Genes Dev. 21: 3369-3380. Garcia BA. et al., 2007, J. Biol. Chem. 282:7641-7655. Yu L.-R. et al., 2007, J. Proteome Res. 6: 4150-4162.

NCBI and Uniprot Product Information

• NCBI GI #: 4504281
• NCBI GeneID: 8350
• NCBI Accession #: NP_003520.1
• NCBI GenBank Nucleotide #: NM_003529.2
• UniProt Accession #: P68431; P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
• Molecular Weight: 15,404 Da
• NCBI Official Full Name: histone H3.1
• NCBI Official Synonym Full Names: histone cluster 1, H3a
• NCBI Official Symbol: HIST1H3A
• NCBI Official Synonym Symbols: H3/A; H3FA
• NCBI Protein Information: histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
• UniProt Protein Name: Histone H3.1
• UniProt Gene Name: HIST1H3A
• UniProt Synonym Gene Names: H3FA
• UniProt Entry Name: H31_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Uniprot Description

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine