SDS-Page
Hepatocyte Growth Factor Activator (HGFAC) Recombinant Protein | HGFAC recombinant protein
Recombinant Hepatocyte Growth Factor Activator (HGFAC)
Gene Names
Hgfac; HGFA
Reactivity
Mus musculus (Mouse)
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Hepatocyte Growth Factor Activator (HGFAC); Recombinant Hepatocyte Growth Factor Activator (HGFAC); HGFAC recombinant protein
Host
E.coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
> 97%
Form/Format
PBS, pH7.4, containing 0.1% SKL, 5% Trehalose.
Concentration
Original Concentration: 70ug/mL (varies by lot)
Sequence
PCRFPFR YGGRMLHSCTSEGSAYRKWC ATTHNYDRDR AWGYCAEVTL PVEGPAILDP CASGPCLNGG TCSSTHDHGSYHCSCPLAFT GKDCGTEKCF DETRYEYFEV GDHWARVSEG HVEQCGCMEG QARCEDTHHTACLSSPCLNG GTCHLIVGTG TSVCTCPLGY AGRFCNIVPT EHCFLGNGTE YRGVASTAASGLSCLAWNSD LLYQELHVDS VAAAVLLGLG PHAYCRNPDK DERPWCYVVK D
Applicable Applications for HGFAC recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Pro104~Asp351
Tags
N-terminal His and GST Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
6.1
Usage
Reconstitute in ddH2O to a concentration of 0-0.5 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 57.0kDa
Accurate Molecular Mass: 57kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 57kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
hepatocyte growth factor activator preproprotein
NCBI Official Synonym Full Names
hepatocyte growth factor activator
NCBI Official Symbol
Hgfac
NCBI Official Synonym Symbols
HGFA
NCBI Protein Information
hepatocyte growth factor activator; HGF activator
UniProt Protein Name
Hepatocyte growth factor activator
Protein Family
UniProt Gene Name
Hgfac
UniProt Synonym Gene Names
HGF activator; HGFA
UniProt Entry Name
HGFA_MOUSE
NCBI Description
This gene encodes a serine protease enzyme that proteolytically activates hepatocyte growth factor (HGF) and plays a vital role in the regulation of HGF activity in the regeneration and repair of various tissues. The encoded protein is an inactive zymogen that is proteolytically activated to generate a heterodimeric enzyme consisting of a short chain and a long chain linked by a disulfide bridge. Mice lacking the encoded protein display an impairment in mucosal regeneration after injury. [provided by RefSeq, Jul 2015]
Uniprot Description
HGFAC: Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. Belongs to the peptidase S1 family.
Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.21.-
Cellular Component: extracellular space; rough endoplasmic reticulum; cytoplasm; extracellular region
Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity
Biological Process: proteolysis
Research Articles on HGFAC
1. HNF1alpha might regulate beta-cell mass or function at least partly by modulating Hgfac expression.
Similar Products
Product Notes
The HGFAC hgfac (Catalog #AAA2011987) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Hepatocyte Growth Factor Activator (HGFAC) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Hepatocyte Growth Factor Activator (HGFAC) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the HGFAC hgfac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PCRFPFR YGGRMLHSCT SEGSAYRKWC ATTHNYDRDR AWGYCAEVTL PVEGPAILDP CASGPCLNGG TCSSTHDHGS YHCSCPLAFT GKDCGTEKCF DETRYEYFEV GDHWARVSEG HVEQCGCMEG QARCEDTHHT ACLSSPCLNG GTCHLIVGTG TSVCTCPLGY AGRFCNIVPT EHCFLGNGTE YRGVASTAAS GLSCLAWNSD LLYQELHVDS VAAAVLLGLG PHAYCRNPDK DERPWCYVVK D. It is sometimes possible for the material contained within the vial of "Hepatocyte Growth Factor Activator (HGFAC), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
