HGF alpha chain Recombinant Protein | HGFA recombinant protein

Human HGF alpha chain Recombinant

Cat. Num. AAA553072

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: HGF alpha chain; Human HGF alpha chain Recombinant; Human Hepatocyte Growth Factor alpha chain Recombinant; hgf; scatter factor; hepatocyte growth factor; Hepatopoietin; hpta; HGFA recombinant protein

• Host: E Coli
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recobinant HGF alpha was lyophilized from a 0.2 um filtered Tris solution pH 8.0.
• Sequence: MWVTKLLPAL LLQHVLLHLL LLPIAIPYAE GQRKRRNTIH EFKKSAKTTL IKIDPALKIK TKKVNTADQC ANRCTRNKGL PFTCKAFVFD KARKQCLWFP FNSMSSGVKK EFGHEFDLYE NKDYIRNCII GKGRSYKGTV SITKSGIKCQ PWSSMIPHEH SFLPSSYRGK DLQENYCRNP RGEEGGPWCF TSNPEVRYEV CDIPQCSEVE CMTCNGESYR GLMDHTESGK ICQRWDHQTP HRHKFLPERY PDKGFDDNYC RNPDGQPRPW CYTLDPHTRW EYCAIKTCAD NTMNDTDVPL ETTECIQGQG EGYRGTVNTI WNGIPCQRWD SQYPHEHDMT PENFKCKDLR ENYCRNPDGS ESPWCFTTDP NIRVGYCSQI PNCDMSHGQD CYRGNGKNYM GNLSQTRSGL TCSMWDKNME DLHRHIFWEP DASKLNENYC RNPDDDAHGP WCYTGNPLIP WDYCPISRCE GDTTPTIVNL DHPV ISCAKT KQLRVVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP >NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS
• Sequence Length: 728

• Domain: Kringles
• Host Note: Optimized DNA sequence encoding Human Hepatocyte Growth Factor alpha chain was expressed in E.Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native human Hepatocyte Growth Factor is generated by the proteolytic removal of the signal peptide, the molecule has a calculated molecular mass of approximately 54 kDa.; Recombinant HGF alpha is a non-glycosylated disulfide-linked protein consisting of the alpha chain (463) amino acid residue subunits, and migrates as an approximately 54 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for HGFA recombinant protein

Hepatocyte growth factor (HGF) is a multifunctional cytokine that was originally described as a mesenchymal- derived factor that regulates cell growth, cell motility, morphogenesis and angiogenesis through activation of its receptor, the transmembrane tyrosine kinase encoded by the c-Met proto-oncogene. HGF and c- Met are often co-expressed or over-expressed in a variety of human malignancies including glioblastoma and medullablastoma. The multifunctional effects of HGF:c-Met signaling in tumor cells are mediated by a network of signal transduction pathways including mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K). HGF consists of two subunits held by a disulfide bond. The alpha subunit (69 kDa) has a length of 440 amino acids. The beta subunit (34 kDa) has a length of 234 amino acids. The beta chain of the factor shows approximately 38 % homology at the protein level to the serine protease domain of plasminogen. A variant of the beta chain has been shown to function as a subunit of a hybrid cytokine known as PPBSF [pre-pro-B- cell growth stimulating factor].

Product Categories/Family for HGFA recombinant protein

Cytokines

References

• HGF family negatively regulates hepatic gluconeogenesis via induction of orphan nuclear receptor shp in primary hepatocytes .J. Biol. Chem., Aug 2009; 10.1074/jbc.M109.022244.
• HGF/met signalling promotes pge2 biogenesis via regulation of cox-2 and 15-pgdh expression in colorectal cancer cells .Carcinogenesis, Aug 2009; 10.1093/carcin/bgp183.
• the angiogenic growth factors HGF and vegf in serum and plasma from neuroblastoma patients .Anticancer Res, Aug 2009; 29: 3311 - 3319.
• possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent sema3a during myogenic differentiation .Am J Physiol Cell Physiol, Aug 2009; 297: C238 - C252.
• HGF and c-met participate in paracrine tumorigenic pathways in head and neck squamous cell cancer .Clin. Cancer Res., Jun 2009; 15: 3740 - 3750.