Human hect domain and RLD 5 ELISA Kit | HERC5 elisa kit

Human E3 ISG15--protein ligase HERC5, HERC5 ELISA Kit

Cat. Num. AAA9329762

• Gene Names: HERC5; CEB1; CEBP1
• Reactivity: Human
• Synonyms: hect domain and RLD 5; Human E3 ISG15--protein ligase HERC5; HERC5 ELISA Kit; Human E3 ISG15--protein ligase HERC5 (HERC5) ELISA kit; CEB1; CEBP1; HECT E3 ubiquitin ligase; HECT domain and RCC1-like domain-containing protein 5; cyclin-E binding protein 1; HERC5 elisa kit

• Reactivity: Human

• Preparation and Storage: Store all reagents at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 110825982
• NCBI GeneID: 51191
• NCBI Accession #: NP_057407.2
• NCBI GenBank Nucleotide #: NM_016323.3
• UniProt Accession #: Q9UII4; Q69G20; B2RTQ1
• Molecular Weight: 116,852 Da
• NCBI Official Full Name: E3 ISG15--protein ligase HERC5
• NCBI Official Synonym Full Names: HECT and RLD domain containing E3 ubiquitin protein ligase 5
• NCBI Official Symbol: HERC5
• NCBI Official Synonym Symbols: CEB1; CEBP1
• NCBI Protein Information: E3 ISG15--protein ligase HERC5; hect domain and RLD 5; cyclin-E-binding protein 1; probable E3 ubiquitin-protein ligase HERC5; HECT domain and RCC1-like domain-containing protein 5
• UniProt Protein Name: E3 ISG15--protein ligase HERC5
• Protein Family: E3 ISG15--protein ligase
• UniProt Gene Name: HERC5
• UniProt Synonym Gene Names: CEB1; CEBP1
• UniProt Entry Name: HERC5_HUMAN

NCBI Description

This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

HERC5: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: ISG15 conjugating enzyme activity; protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ISG15-protein conjugation; cytokine and chemokine mediated signaling pathway; regulation of defense response to virus; protein ubiquitination during ubiquitin-dependent protein catabolic process; innate immune response; regulation of cyclin-dependent protein kinase activity; negative regulation of interferon type I production; defense response to virus