Goat Hepcidin ELISA Kit | HEPC elisa kit

Goat Hepcidin ELISA Kit

Cat. Num. AAA44535

• Gene Names: HAMP; HEPC; PLTR; HFE2B; LEAP1
• Reactivity: Goat
• Synonyms: Hepcidin; Goat Hepcidin ELISA Kit; HEPC elisa kit

• Reactivity: Goat
• Specificity: No significant cross-reactivity or interference between Goat HEPC and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 6.25 ng/ml - 200 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated HEPC concentrations in Goat serum, plasma and other body fluids. Using Purified Goat HEPC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HEPC and HEPC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HEPC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for HEPC elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated HEPC concentrations in Goat serum, plasma and other body fluids. Using Purified Goat HEPC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HEPC and HEPC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HEPC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10863973
• NCBI GeneID: 57817
• NCBI Accession #: NP_066998.1
• NCBI GenBank Nucleotide #: NM_021175.2
• UniProt Accession #: P81172; Q1HE14; Q9BY68
• Molecular Weight: 9,408 Da
• NCBI Official Full Name: hepcidin preproprotein
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: HAMP
• NCBI Official Synonym Symbols: HEPC; PLTR; HFE2B; LEAP1
• NCBI Protein Information: hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1
• UniProt Protein Name: Hepcidin
• Protein Family: Hepcidin
• UniProt Gene Name: HAMP
• UniProt Synonym Gene Names: HEPC; LEAP1; LEAP-1; PLTR; Hepc25; Hepc20
• UniProt Entry Name: HEPC_HUMAN

NCBI Description

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]

Uniprot Description

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: apical cortex; extracellular region

Molecular Function: hormone activity

Biological Process: cellular iron ion homeostasis; killing of cells of another organism; defense response to bacterium; immune response; defense response to fungus

Disease: Hemochromatosis, Type 2b