Hepatitis C Virus Core, 22kD Recombinant Protein
Hepatitis C Virus Core, 22kD, Recombinant (HCV)
Applications
ELISA, Western Blot
Purity
Affinity Purified~90% by SDS-PAGE, reduced. Purified by immunoaffinity chromatography methods.
Synonyms
Hepatitis C Virus Core; 22kD; Recombinant (HCV); 22kD recombinant protein
Host
Pichia pastoris
Specificity
This protein binds murine monoclonal antibodies and HCV converted human serum polyclonal antibodies in ELISA and Western Blot.
Purity/Purification
Affinity Purified
~90% by SDS-PAGE, reduced. Purified by immunoaffinity chromatography methods.
~90% by SDS-PAGE, reduced. Purified by immunoaffinity chromatography methods.
Form/Format
Supplied as a liquid in 50mM Tris, pH 8.0, 0.1mM sodium chloride. No stabilizers or preservatives added.
Sequence
1 mstnpkpqrk tkrntnrrpq dvkfpgggqi vggvyllprr gprlgvratr ktsersqprg
61 rrqpipkarr pegrtwaqpg ypwplygneg cgwagwllsp rgsrpswgpt dprrrsrnlg
121 kvidtltcgf adlmgyiplv gaplggaara lahgvrvled gvnyatgnlp gcsfsiflla
181 llscltvpas a
61 rrqpipkarr pegrtwaqpg ypwplygneg cgwagwllsp rgsrpswgpt dprrrsrnlg
121 kvidtltcgf adlmgyiplv gaplggaara lahgvrvled gvnyatgnlp gcsfsiflla
181 llscltvpas a
Applicable Applications for Hepatitis C Virus Core, 22kD recombinant protein
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Dilution: solid phase antigen-antibody titration assay:
Dilute the antigen preparation 10 to 20-fold in PBS.
Western Blot:
10ng range depending on the nature and affinity of the secondary detection reagent.
ELISA:
HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions.
Dilute the antigen preparation 10 to 20-fold in PBS.
Western Blot:
10ng range depending on the nature and affinity of the secondary detection reagent.
ELISA:
HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Hepatitis C Virus Core, 22kD recombinant protein
Hepatitis C full length nucleocapsid recombinant protein (p22) produced in Pichia pastoris expression system.
Product Categories/Family for Hepatitis C Virus Core, 22kD recombinant protein
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Product Notes
The Hepatitis C Virus Core, 22kD (Catalog #AAA637073) is a Recombinant Protein produced from Pichia pastoris and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hepatitis C Virus Core, 22kD can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Dilution: solid phase antigen-antibody titration assay: Dilute the antigen preparation 10 to 20-fold in PBS. Western Blot: 10ng range depending on the nature and affinity of the secondary detection reagent. ELISA: HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions. Researchers should empirically determine the suitability of the Hepatitis C Virus Core, 22kD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 1 mstnpkpqrk tkrntnrrpq dvkfpgggqi vggvyllprr gprlgvratr ktsersqprg 61 rrqpipkarr pegrtwaqpg ypwplygneg cgwagwllsp rgsrpswgpt dprrrsrnlg 121 kvidtltcgf adlmgyiplv gaplggaara lahgvrvled gvnyatgnlp gcsfsiflla 181 llscltvpas a. It is sometimes possible for the material contained within the vial of "Hepatitis C Virus Core, 22kD, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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