Hepatitis B Virus X-protein Recombinant Protein
Hepatitis B Virus X-protein, Recombinant (trans-activator X Gene Product)
Purity
Highly Purified95%; Two-step procedure using size exclusion chromatography before and after refolding.
Synonyms
Hepatitis B Virus X-protein; Recombinant (trans-activator X Gene Product); Hepatitis B Virus X-protein recombinant protein
Host
E Coli
Purity/Purification
Highly Purified
95%; Two-step procedure using size exclusion chromatography before and after refolding.
95%; Two-step procedure using size exclusion chromatography before and after refolding.
Form/Format
Supplied as a lyophilized powder from 0.05M acetate buffer, pH 4.0.
Sequence
17kD protein containing 154 amino acid residues. MAARVCCQLD PARDVLCLRP VGAESRGRPV SGPFGTLPSP SSSAVPADHG AHLSLRGLPV CAFSSAGPCA LRFTSARRME TTVNAHQVLP KVLHKRTLGL SAMSTTDLEA YFKDCLFKDW EELGEEIRLK VFVLGGCRHK LVCSPAPCNF FTSA.
Application Notes
Suitable for use in ELISA and Western Blot.
Species Sequence Homology
Native HBx: 100%
Reconstitution
Reconstitute with 0.1M Acetate buffer, pH 4.0 to a concentration of 0.5mg/ml. For conversion into a higher pH value, intensive dilution by relevant buffer to a concentraion of 10ug/ml is recommended. (In higher concentrations, the solubility of the antigen is limited.)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding 0.1M Acetate buffer. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer,
Related Product Information for Hepatitis B Virus X-protein recombinant protein
Hepatitis B virus X protein (HBx) is a 17kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. rHBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. Recombinant HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.
Product Categories/Family for Hepatitis B Virus X-protein recombinant protein
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Product Notes
The Hepatitis B Virus X-protein (Catalog #AAA637669) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the Hepatitis B Virus X-protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 17kD protein containing 154 amino acid residues. MAARVCCQLD PARDVLCLRP VGAESRGRPV SGPFGTLPSP SSSAVPADHG AHLSLRGLPV CAFSSAGPCA LRFTSARRME TTVNAHQVLP KVLHKRTLGL SAMSTTDLEA YFKDCLFKDW EELGEEIRLK VFVLGGCRHK LVCSPAPCNF FTSA. It is sometimes possible for the material contained within the vial of "Hepatitis B Virus X-protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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