Immunofluorescence
(Immunofluorescent staining of NeuN antibodyCA1 region of a transgenic mouse expressing the eGFP gene product under control of the Thy-1 promoter.Green is Egfp autofluorescence; Red is Cy-3 secondary antibody labeling chicken anti-Neu-N (Fox-3) positive neuronal nuclei. Picture courtesy of Dr. Felix Eckenstein, University of Vermont. )
Hens NeuN Antibody | anti-NeuN antibody
NeuN antibody
Gene Names
Rbfox3; NeuN; Fox-3; Hrnbp3; Neuna60
Applications
Immunocytochemistry, Immunohistochemistry
Purity
NeuN antibody was purified by affinity chromatography.
Synonyms
NeuN; NeuN antibody; Polyclonal NeuN; Anti-NeuN; Fox 3; Fox-3; Neu-N; Neu N; anti-NeuN antibody
Host
Hens
Purity/Purification
NeuN antibody was purified by affinity chromatography.
Form/Format
Phosphate buffered (10mM) isotonic (0.9%, w/v) saline (PBS, pH7.2) with 50% (v/v) glycerol and 0.02% sodium azide as preservative.
Concentration
100 ug/ml (varies by lot)
Applicable Applications for anti-NeuN antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
Usage Recommendations:
ICC: 1:2,000 - 1:5,000
IHC: 11:2,000 - 1:5,000
ICC: 1:2,000 - 1:5,000
IHC: 11:2,000 - 1:5,000
Immunogen
Synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate.
Preparation and Storage
Store at 4°C. PROTECT FROM LIGHT.
(Note: Each time an antibody preparation is frozen, about half its binding activity is lost.)
(Note: Each time an antibody preparation is frozen, about half its binding activity is lost.)
Immunofluorescence
(Immunofluorescent staining of NeuN antibodyCA1 region of a transgenic mouse expressing the eGFP gene product under control of the Thy-1 promoter.Green is Egfp autofluorescence; Red is Cy-3 secondary antibody labeling chicken anti-Neu-N (Fox-3) positive neuronal nuclei. Picture courtesy of Dr. Felix Eckenstein, University of Vermont. )
Immunofluorescence
(Immunofluorescent staining of NeuN antibodyCA1 region of a transgenic mouse expressing the eGFP gene product under control of the Thy-1 promoter.Green is Egfp autofluorescence; Red is Cy-3 secondary antibody labeling chicken anti-Neu-N (Fox-3) positive neuronal nuclei. Picture courtesy of Dr. Felix Eckenstein, University of Vermont. )
Related Product Information for anti-NeuN antibody
Description: Affinity purified Chicken polyclonal NeuN antibody
Biological Significance: The vast majority of neurons are strongly NeuN positive, and NeuN immunoreactivity has been widely used to identify neurons is tissue culture and in sections and to measure the neuron/glial ratio in brain regions. NeuN immunoreactivity becomes obvious as neurons mature, typically after they have downregulated expression of Doublecortin, a marker seen in the earliest stages of neuronal development.
Biological Significance: The vast majority of neurons are strongly NeuN positive, and NeuN immunoreactivity has been widely used to identify neurons is tissue culture and in sections and to measure the neuron/glial ratio in brain regions. NeuN immunoreactivity becomes obvious as neurons mature, typically after they have downregulated expression of Doublecortin, a marker seen in the earliest stages of neuronal development.
Product Categories/Family for anti-NeuN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
RNA binding protein fox-1 homolog 3 isoform 3
NCBI Official Synonym Full Names
RNA binding protein, fox-1 homolog (C. elegans) 3
NCBI Official Symbol
Rbfox3
NCBI Official Synonym Symbols
NeuN; Fox-3; Hrnbp3; Neuna60
NCBI Protein Information
RNA binding protein fox-1 homolog 3
UniProt Protein Name
RNA binding protein fox-1 homolog 3
UniProt Gene Name
Rbfox3
UniProt Synonym Gene Names
D11Bwg0517e; Hrnbp3; Fox-3; NeuN
UniProt Entry Name
RFOX3_MOUSE
Uniprot Description
NeuN: RNA-binding protein that regulates alternative splicing events.
Protein type: RNA processing; DNA-binding; RNA splicing
Cellular Component: cell soma; cytoplasm; perikaryon; intracellular; nucleus
Molecular Function: protein binding; DNA binding; nucleic acid binding; RNA binding; nucleotide binding
Biological Process: regulation of RNA splicing; RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on NeuN
Similar Products
Product Notes
The NeuN rbfox3 (Catalog #AAA837654) is an Antibody produced from Hens and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NeuN can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC). Usage Recommendations: ICC: 1:2,000 - 1:5,000 IHC: 11:2,000 - 1:5,000. Researchers should empirically determine the suitability of the NeuN rbfox3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NeuN, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
