Avidin Active Protein | AVD active protein
Avidin (Egg White, 68kD Glycoprotein)
Purity
Affinity PurifiedPurified by affinity chromatographic techniques.
Synonyms
Avidin; Avidin (Egg White; 68kD Glycoprotein); AVD active protein
Host
Hen egg white
Purity/Purification
Affinity Purified
Purified by affinity chromatographic techniques.
Purified by affinity chromatographic techniques.
Form/Format
Supplied as a lyophilized powder.
Application Notes
Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.
Activity
Greater than 11 U/mg, 1 unit binds 1µg biotin.
Endotoxin Level
0.1ng/ug (IEU/ug) of Avidin.
Solubility
It is recommended to reconstitute the lyophilized Avidin in sterile 18M -cm H2O not less than 100µg/ml or more than 10mg/ml. solutions
Preparation and Storage
-20 degree C
Stability: Avidin although stable at 40C for 3 weeks, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Stability: Avidin although stable at 40C for 3 weeks, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Related Product Information for AVD active protein
Avidin is a glycosylated polypeptide chain having a molecular mass of 68kD and containg 4 subunits each with a binding site for biotin. Avidin is purified by affinity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB).
Product Categories/Family for AVD active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68kD
NCBI Official Full Name
avidin
NCBI Official Synonym Full Names
avidin
NCBI Official Symbol
AVD
NCBI Protein Information
avidin; mature avidin
UniProt Protein Name
Avidin
Protein Family
UniProt Gene Name
AVD
UniProt Entry Name
AVID_CHICK
Uniprot Description
Function: The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
Subunit structure: Homotetramer.
Subcellular location: Secreted.
Tissue specificity: Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
Post-translational modification: N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
Sequence similarities: Belongs to the avidin/streptavidin family.Contains 1 avidin-like domain.
Similar Products
Product Notes
The AVD avd (Catalog #AAA634712) is an Active Protein produced from Hen egg white and is intended for research purposes only. The product is available for immediate purchase. Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques. Researchers should empirically determine the suitability of the AVD avd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Avidin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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